[In vitro combination of mercaptopurine and human serum albumin].

Abstract

Through the use of the fluorescence absorbance technique, the parameters that define the association between the antineoplastic drug mercaptopurine and human serum albumin were determined for a concentration range for the ligand between 6 X 10(-5) and 10(-7) moles/L, keeping the concentration of the macromolecule fixed at 0.5 X 10(-6) moles/l. Given the structure of the macromolecule under study and the experimental conditions used, we established an association model with two different kinds of binding sites.