Decellularized skin enriched with methacrylated hyaluronic acid enhances cell adhesion and accelerates wound closure

IF 6.1 3区医学 Q1 MATERIALS SCIENCE, BIOMATERIALS

Biomaterials Science Pub Date : 1900-01-01 DOI:10.1039/d6bm01132b

Platania, Varvara; Tsanaktsidou, Evgenia; Kammona, Olga; Chatzinikolaidou, Maria

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引用次数: 0

Abstract

Dermal substitutes that simultaneously provide structural fidelity and bioactive signaling remain limited in clinical wound care. Here, we report a hybrid dermal patch based on decellularized bovine dermis (dDerm) coated with photo-crosslinked methacrylated hyaluronic acid (MeHA-Derm). Successful functionalization was confirmed by FTIR and SEM, demonstrating formation of a uniform hydrogel layer. The MeHA-Derm patches exhibited enhanced swelling capacity and improved mechanical performance, with Young's modulus significantly increasing relative to native bovine skin. HaCaT keratinocytes cultured on MeHA-Derm showed high cytocompatibility, with viability exceeding 90% at days 3 and 5. Metabolic activity was significantly elevated in MeHA-Derm compared to dDerm. Immunofluorescence revealed increased vinculin secretion, indicating stronger focal adhesion. In vitro scratch assay demonstrated a significantly accelerated wound closure of 82 ± 12% on day 5 on MeHA-Derm versus dDerm at 43 ± 25%. Moreover, ex vivo analysis confirmed superior gap closure, reaching 92 ± 4% by day 10 compared to 84 ± 14% on dDerm. Overall, the MeHA coating enhances hydration, mechanical stability, and cell–matrix interactions while preserving native dermal architecture. This strategy provides a scalable and biologically instructive platform for next-generation skin regeneration and advanced wound care applications.

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富含甲基丙烯酸透明质酸的去细胞皮肤，增强细胞粘附，加速伤口愈合。

同时提供结构保真度和生物活性信号的真皮替代品在临床伤口护理中仍然有限。在这里，我们报告了一种基于脱细胞牛真皮（dDerm）涂有光交联甲基丙烯酸透明质酸（MeHA-Derm）的杂交皮肤贴片。通过红外光谱（FTIR）和扫描电镜（SEM）证实了功能化的成功，表明形成了均匀的水凝胶层。MeHA-Derm贴片具有增强的肿胀能力和改善的机械性能，相对于天然牛皮肤，杨氏模量显着增加。在MeHA-Derm上培养的HaCaT角质形成细胞表现出较高的细胞相容性，在第3天和第5天的存活率超过90%。与dDerm相比，MeHA-Derm的代谢活性显著升高。免疫荧光显示血管素分泌增加，提示病灶黏附增强。体外划痕实验显示，MeHA-Derm在第5天显著加速伤口愈合，速度为82±12%，而dDerm为43±25%。此外，离体分析证实了更优越的间隙闭合，第10天达到92±4%，而dDerm为84±14%。总的来说，MeHA涂层增强了水合作用、机械稳定性和细胞-基质相互作用，同时保留了天然的真皮结构。该策略为下一代皮肤再生和高级伤口护理应用提供了可扩展和生物学指导平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomaterials Science MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

11.50

自引率

4.50%

发文量

556

期刊介绍： Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions.