Culturable Endophytic Fungi of Atractylodes macrocephala: Influence of Tissue Type and Geographic Location on Their Antifungal, Therapeutic, and Cytotoxic Potentials.

IF 2.8 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2026-10-21 DOI:10.1007/s00284-026-05163-y

Yuzhe Li, Yueqing Hong, Kaohua Liu, Yixue Ding, Hao Zheng, Linxin Ye, Siyuan Zhang, Qinger Huang, Jiangnan Shi, Jingyi Xu, Shaokai Xu, Luping Qin, Bo Zhu

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Abstract

Atractylodes macrocephala Koidz. is a medicinal plant that has been widely used as a tonic agent in different ethno-medical systems in East Asia. This study explores the culturable endophytic fungi of A. macrocephala and potential correlations between these fungi and the active components of this medicinal plant. The antipathogenic activity of these endophytic fungi against three species of pathogenic Fusarium was examined. The cytotoxicity of the endophytic fungi extracts was assessed in five human cancer cell lines. A total of 2338 endophytic fungal isolates were obtained from the root, stem, leaf, and rhizome of A. macrocephala. The endophytic fungal community structure in A. macrocephala was influenced by tissue type and geographical location. Trichothecium roseum (Pers.) Link was found to be closely associated with atractylenolide I, a major active component of A. macrocephala. In total, 40 of the 77 endophytic fungal taxa (51.95%) manifested inhibitory effects on the growth of at least one of the fungal pathogens. In addition, metabolites from most fungal endophytes exhibited cytotoxicity against the cancer cells; in particular, AM450 and AM448 were the most potent (IC₅₀ < 5 μg/mL) against MCF-7, A549, KB, KB-VIN, and MDA-MB-231. These extracts showed potent cytotoxicity against cancer cell lines and warrant further study; toxicity testing on normal cells is needed to assess selectivity and therapeutic potential.

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白术可培养内生真菌：组织类型和地理位置对其抗真菌、治疗和细胞毒性的影响。

苍术。是一种药用植物，在东亚不同民族的医疗体系中被广泛用作滋补剂。本研究探讨了大头草可培养的内生真菌及其与该药用植物活性成分的潜在相关性。研究了这些内生真菌对3种致病性镰刀菌的抑菌活性。研究了内生真菌提取物在5种人类癌细胞系中的细胞毒性。从大头藤根、茎、叶和根茎中分离得到内生真菌2338株。组织类型和地理位置对大头马内生真菌群落结构有影响。玫瑰毛霉发现Link与白术内酯I密切相关，白术内酯I是白术的主要活性成分。在77个内生真菌分类群中，有40个（51.95%）对至少一种真菌病原菌的生长表现出抑制作用。此外，大多数真菌内生菌的代谢物对癌细胞表现出细胞毒性；特别是，AM450和AM448对MCF-7， A549， KB， KB- vin和MDA-MB-231最有效（IC₅₀< 5 μg/mL）。这些提取物对癌细胞具有较强的细胞毒性，值得进一步研究；需要对正常细胞进行毒性试验，以评估其选择性和治疗潜力。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.