Response Surface Optimization of Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Polysaccharides From Sword Bean (Canavalia gladiata (Jacq.) DC.) Pods.

IF 2.9 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry & Biodiversity Pub Date : 2026-10-01 DOI:10.1002/cbdv.71766

Jieyu Xing, Linjing Zhang, Zhaoxuan Liang, Zheng Liu, Haiyun Chen, Xian Fu

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Abstract

Safe and functional natural active ingredients are a research hotspot in early intervention studies of chronic diseases. This study focuses on a polysaccharide (CGPs‑1) from sword bean (Canavalia gladiata (Jacq.) DC.) pods. Its optimal ultrasound-assisted extraction conditions were achieved with choline chloride/oxalic acid DES (2:1), 34% water, 21 mL/g, 65°C, 29 min at 280 W; the actual yield (33.24%) was consistent with the predicted value (33.96%). CGPs‑1 was identified as a pyran‑type polysaccharide, comprising rhamnose (Rha), glucuronic acid (GlcA), and galacturonic acid (GalA) in a molar ratio of 1.08:1:1.78. CGPs‑1 had IC₅₀ values of 0.157 mg/mL (α‑glucosidase), 0.083 mg/mL (α‑amylase), 31.18 mg/mL (DPPH radicals), and 2.264 mg/mL (ABTS radicals), inhibited LPS‑triggered inflammatory response and LPS‑mediated neurotoxicity in BV2 cells, and also suppressed MPTP-induced inflammatory response in zebrafish larvae. Results indicate that CGPs‑1 exhibits antioxidant, hypoglycemic, anti‑neuroinflammatory and neuroprotective potentials, providing preliminary experimental support for further research and the development of functional‑food‑related products derived from sword bean pods.

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超声辅助深度共晶溶剂萃取剑豆生物活性多糖的响应面优化直流)。豆荚。

安全、功能性天然活性成分是慢性病早期干预研究的研究热点。本研究重点从剑豆（Canavalia gladiata (Jacq.)）中提取多糖（CGPs - 1）。直流)豆荚。超声辅助提取的最佳条件为：氯化胆碱/草酸DES(2:1)， 34%水，21 mL/g, 65℃，29 min, 280 W；实际产率（33.24%）与预测值（33.96%）基本一致。cpps‑1被鉴定为吡喃型多糖，由鼠李糖（Rha）、葡萄糖醛酸（GlcA）和半乳糖醛酸（GalA）组成，摩尔比为1.08:1:1.78。CGPs - 1的IC50值分别为0.157 mg/mL （α -葡萄糖苷酶）、0.083 mg/mL （α -淀粉酶）、31.18 mg/mL （DPPH自由基）和2.264 mg/mL （ABTS自由基），可抑制LPS引发的炎症反应和LPS介导的BV2细胞神经毒性，也可抑制mptp诱导的斑马鱼幼鱼炎症反应。结果表明，CGPs - 1具有抗氧化、降血糖、抗神经炎症和神经保护作用，为进一步研究和开发剑豆荚衍生的功能性食品相关产品提供了初步的实验支持。

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来源期刊

Chemistry & Biodiversity 环境科学-化学综合

CiteScore

3.40

自引率

10.30%

发文量

475

审稿时长

2.6 months

期刊介绍： Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.