Longitudinal profiling of host- and microbe-derived lipid metabolites in human breast milk

IF 2.1 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Early human development Pub Date : 2027-01-01 Epub Date: 2026-10-14 DOI:10.1016/j.earlhumdev.2026.106697

Masashi Hotta, Koji Hosomi, Keita Uchiyama, Mari Furuta, Yoko Tojima, Shigenobu Kishino, Noriko Shibuya, Jun Ogawa, Jun Kunisawa

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引用次数: 0

Abstract

Objective

Breast milk contains lipid metabolites and is indispensable for the growth and development of neonates and infants. However, studies investigating interindividual variations in human breast milk composition and the longitudinal changes in its constituent lipid metabolites remain limited. The temporal and individual patterns of variations in these metabolites were characterized to provide a foundation for future research on precision nutrition in infants.

Study design

Samples of human breast milk were collected shortly after birth and at 1 and 6 months postpartum, from participants who delivered healthy, full-term infants. Lipidomic analysis involving liquid chromatography coupled with tandem-mass spectrometry was used to quantify the levels of host- and microbe-derived fatty acid metabolites in the samples. The population was classified into five groups and analyzed based on the distribution of fatty acid substrate at 0 months.

Results

Breast milk samples from 116 participants were analyzed. The group-specific metabolite profiles were classified into three distinct patterns. The concentrations of substrates and many metabolites increased from birth to 1 month, particularly among samples with lower baseline concentrations, whereas from 1 to 6 months, some metabolites remained stable, while others decreased. These patterns were associated with differences in the metabolic pathways.

Conclusions

Longitudinal lipid metabolites, including microbe-derived metabolites, in human breast milk and presented characteristic patterns. These findings provide foundational data for future research on precision nutrition for infants based on the metabolic profiles of breast milk.

查看原文本刊更多论文

人母乳中宿主和微生物衍生的脂质代谢物的纵向分析。

目的：母乳中含有脂质代谢物，是新生儿和婴幼儿生长发育不可缺少的营养物质。然而，调查人类母乳成分的个体间差异及其组成脂质代谢物的纵向变化的研究仍然有限。这些代谢物变化的时间和个体模式被表征，为婴儿精确营养的未来研究提供基础。研究设计：在出生后不久以及产后1个月和6个月 时，从分娩健康足月婴儿的参与者中收集人类母乳样本。脂质组学分析包括液相色谱联用串联质谱法来量化样品中宿主和微生物来源的脂肪酸代谢物的水平。根据0 月脂肪酸底物的分布情况，将种群分为5组进行分析。结果：对116名参与者的母乳样本进行了分析。组特异性代谢物谱分为三种不同的模式。从出生到1 个月，底物和许多代谢物的浓度增加，特别是在基线浓度较低的样品中，而从1到6 个月，一些代谢物保持稳定，而另一些代谢物则下降。这些模式与代谢途径的差异有关。结论：人母乳中的纵向脂质代谢物，包括微生物来源的代谢物，呈现出特有的模式。这些发现为未来基于母乳代谢谱的婴儿精准营养研究提供了基础数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Early human development 医学-妇产科学

CiteScore

4.40

自引率

4.00%

发文量

100

审稿时长

46 days

期刊介绍： Established as an authoritative, highly cited voice on early human development, Early Human Development provides a unique opportunity for researchers and clinicians to bridge the communication gap between disciplines. Creating a forum for the productive exchange of ideas concerning early human growth and development, the journal publishes original research and clinical papers with particular emphasis on the continuum between fetal life and the perinatal period; aspects of postnatal growth influenced by early events; and the safeguarding of the quality of human survival. The first comprehensive and interdisciplinary journal in this area of growing importance, Early Human Development offers pertinent contributions to the following subject areas: Fetology; perinatology; pediatrics; growth and development; obstetrics; reproduction and fertility; epidemiology; behavioural sciences; nutrition and metabolism; teratology; neurology; brain biology; developmental psychology and screening.