Pathogen-targeted quick multiplex PCR identification of Chlamydia psittaci and Haemophilus influenzae coinfection in pneumonia: a case report.

IF 3.6 3区医学 Q1 MEDICINE, GENERAL & INTERNAL

Frontiers in Medicine Pub Date : 2026-10-05 eCollection Date: 2026-01-01 DOI:10.3389/fmed.2026.1890644

Ying Yuan, Jianhong Wang, Ning Feng, Fen Wu, Xiao Dong, Youxin Xu, Shunfeng Zhao, Tingzhao Tan

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Abstract

This study describes a rare case of pneumonia induced by coinfection with Chlamydia psittaci (C. psittaci) and Haemophilus influenzae (H. influenzae). The patient had a history of chronic nephritis and long-term pigeon breeding. The initial manifestation was slurred speech, followed by rapid progression to high fever and respiratory failure. Chest computed tomography revealed bilateral interstitial pneumonia, predominantly in the right lung. Pathogen-targeted quick multiplex polymerase chain reaction (pt-qPCR) confirmed the pathogens as C. psittaci and H. influenzae, and metagenomic next-generation sequencing (mNGS) further verified consistent results. After 7-days of combination therapy with doxycycline and azithromycin, the treatment was switched to doxycycline combined with moxifloxacin for an additional 8-days. The patient achieved complete recovery and was discharged from the hospital. This case suggests that pt-qPCR may facilitate early identification of mixed infections in patients with severe pneumonia and a history of avian exposure, thereby providing valuable information to guide clinical decision-making.

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肺炎中鹦鹉热衣原体和流感嗜血杆菌合并感染的病原体靶向快速多重PCR鉴定1例。

本研究描述了一例罕见的由鹦鹉热衣原体（鹦鹉热衣原体）和流感嗜血杆菌（流感嗜血杆菌）共同感染引起的肺炎。患者有慢性肾炎病史，长期养鸽。最初表现为言语不清，随后迅速发展为高烧和呼吸衰竭。胸部电脑断层显示双侧间质性肺炎，以右肺为主。病原菌靶向快速多聚酶链反应（pt-qPCR）证实病原菌为鹦鹉热锥虫和流感嗜血杆菌，新一代宏基因组测序（mNGS）进一步验证了结果的一致性。在多西环素和阿奇霉素联合治疗7天后，改为多西环素和莫西沙星联合治疗8天。病人完全康复出院。该病例提示，pt-qPCR可能有助于早期识别重症肺炎和禽类暴露史患者的混合感染，从而为指导临床决策提供有价值的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Medicine Medicine-General Medicine

CiteScore

5.10

自引率

5.10%

发文量

3710

审稿时长

12 weeks

期刊介绍： Frontiers in Medicine publishes rigorously peer-reviewed research linking basic research to clinical practice and patient care, as well as translating scientific advances into new therapies and diagnostic tools. Led by an outstanding Editorial Board of international experts, this multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. In addition to papers that provide a link between basic research and clinical practice, a particular emphasis is given to studies that are directly relevant to patient care. In this spirit, the journal publishes the latest research results and medical knowledge that facilitate the translation of scientific advances into new therapies or diagnostic tools. The full listing of the Specialty Sections represented by Frontiers in Medicine is as listed below. As well as the established medical disciplines, Frontiers in Medicine is launching new sections that together will facilitate - the use of patient-reported outcomes under real world conditions - the exploitation of big data and the use of novel information and communication tools in the assessment of new medicines - the scientific bases for guidelines and decisions from regulatory authorities - access to medicinal products and medical devices worldwide - addressing the grand health challenges around the world