Discovery of a highly active condition-responsive promoter for constructing a C1 gas-fermenting platform based on acetogen, Eubacterium callanderi KIST612

IF 8.2 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-10-19 DOI:10.1016/j.biortech.2026.136069

Byeongchan Kang, Ji-Yeon Kim, Soyoung Oh, Minji Kim, Duleepa Pathiraja, In-Geol Choi, In Seop Chang

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引用次数: 0

Abstract

Integrating differential RNA-seq profiling with de novo motif discovery in Eubacterium callanderi KIST612 identified distinct sequence features associated with substrate-responsive transcription. Specifically, MOTIF4 and MOTIF17 showed significant correlations with glucose- and C1 gas (CO)-responsive gene expression, respectively. Based on these motif profiles, a candidate CO-responsive promoter, CI04, was selected and experimentally validated in vivo using a β-glucuronidase (GUS) reporter assay. CI04 exhibited specific CO-responsive activity, exceeding 500 mU mg⁻¹ under CO conditions. Under CO conditions, CI04 activity was approximately 10-fold higher than under glucose conditions, 59-fold higher than that of the constitutive (CT) promoter, and approximately 2.5-fold higher under CO conditions than that of the H2 promoter previously used for overexpression in E. callanderi KIST612.

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基于卡氏真杆菌KIST612构建C1气体发酵平台的高活性条件响应启动子的发现。

将卡氏真杆菌KIST612的差异RNA-seq分析与从头基序发现相结合，鉴定出与底物响应性转录相关的不同序列特征。具体而言，MOTIF4和MOTIF17分别与葡萄糖和C1气体（CO）应答基因表达显著相关。基于这些基序，我们选择了一个候选的co响应启动子CI04，并使用β-葡萄糖醛酸酶（GUS）报告基因法在体内进行了实验验证。在CO条件下，CI04表现出特定的CO响应活性，超过500 mU mg-1。在CO条件下，CI04的活性比葡萄糖条件下高约10倍，比组成子（CT）启动子高59倍，比之前用于callanderi KIST612过表达的H2启动子高约2.5倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.