Creation of fragrant wheat by targeted mutagenesis of either BADH1 or both BADH1 and BADH2 using CRISPR/Cas9.

Abstract

Editing TaBADH1 alone or TaBADH1 and TaBADH2 via CRISPR/Cas9 produced fragrant wheat with up to 11.24-fold higher 2-acetyl-1-pyrroline than the wild type without yield penalty, revealing asymmetric functional redundancy between these two genes and providing elite germplasm for wheat quality improvement.