{"title":"Identification of GEI-14 as a candidate pro-crossover factor by TurboID in <i>C. elegans</i>.","authors":"Yuxin Xi, Yifei Zhao, Ye Hong, Hongtao Zhang","doi":"10.17912/micropub.biology.002380","DOIUrl":null,"url":null,"abstract":"<p><p>Previously, our lab used TurboID-based proximity labeling followed by mass spectrometry (MS) to identify proteins that interact with COSA-1 , a cyclin-like pro-crossover factor that localizes at meiotic crossover sites. Here, we investigated potential interactions of COSA-1 with the top 10 COSA-1 ::TurboID-MS candidates ranked by relative intensity. Beyond the established COSA-1 - CDK-2 interaction, AlphaFold 3 predicted strong binding solely between COSA-1 and GEI-14 . Yeast two-hybrid assays confirmed this interaction and further revealed binding of GEI-14 to MSH-5 , another pro-crossover factor. These results demonstrate TurboID's utility in studying the pro-crossover interactome and highlight GEI-14 as a promising candidate for further characterization.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675796/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.002380","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Previously, our lab used TurboID-based proximity labeling followed by mass spectrometry (MS) to identify proteins that interact with COSA-1 , a cyclin-like pro-crossover factor that localizes at meiotic crossover sites. Here, we investigated potential interactions of COSA-1 with the top 10 COSA-1 ::TurboID-MS candidates ranked by relative intensity. Beyond the established COSA-1 - CDK-2 interaction, AlphaFold 3 predicted strong binding solely between COSA-1 and GEI-14 . Yeast two-hybrid assays confirmed this interaction and further revealed binding of GEI-14 to MSH-5 , another pro-crossover factor. These results demonstrate TurboID's utility in studying the pro-crossover interactome and highlight GEI-14 as a promising candidate for further characterization.
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