Iron biofortification and increased resistance level to leaf rust of spring wheat lines by mutation of vacuolar iron transporters, VIT.

IF 3.6 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2026-10-01 Epub Date: 2026-09-11 DOI:10.1007/s12298-026-01812-5

Aizhan Beisenova, Saule Kenzhebayeva, Alfia Abekova, Saule Atabayeva, Nargul Omirbekova, Ludmila Ignatova, Aigul Amirova, Adel Rsatayeva, Albrecht Serfling

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引用次数: 0

Abstract

Leaf rust (LR), caused by the obligate biotrophic fungus Puccinia triticina Eriks., is a major disease threatening wheat productivity and grain nutritional quality worldwide. This study evaluated advanced M₄ mutant lines of spring wheat generated by gamma irradiation (250-350 Gy) of cv. Kazakhstanskaya-10 to investigate the relationship between LR resistance and vacuolar iron (Fe) transport. Phenotypic and semi-quantitative histological analyses identified highly resistant mutant lines exhibiting both seedling and adult-plant resistance. Resistant genotypes displayed an early pre-haustorial hypersensitive response that was absent in the susceptible wild-type. Gene-targeted sequence analysis of the TaVIT4-D vacuolar iron transporter locus (XM_044518495.1) revealed a polymorphism-rich region characterized by an elevated frequency of transition and transversion events. Transmembrane topology prediction and hydropathy analyses indicated that several non-synonymous, charge-altering amino-acid substitutions were localized within predicted cytosolic Fe-binding regions between TMH2 and TMH3, altering local physicochemical properties while preserving the overall transmembrane architecture. These mutations were associated with increased grain Fe accumulation, with the most resistant mutant line exhibiting a 2.11-fold increase in grain Fe concentration relative to the wild-type. Collectively, the findings support a potential role of TaVIT4-D variation in coordinating LR resistance and grain Fe accumulation and are consistent with a model involving Fe-withholding nutritional immunity and oxidative defense. The results suggest that induced mutagenesis affectingTaVIT4-D may represent a promising non-transgenic strategy for improving both disease resistance and grain micronutrient content in wheat.

Supplementary information: The online version contains supplementary material available at https://doi.org/10.1007/s12298-026-01812-5.

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液泡铁转运蛋白VIT突变对铁生物强化及提高春小麦抗叶锈病水平的影响。

由专性生物营养真菌小麦锈菌引起的叶锈病（LR）。稻瘟病是世界范围内威胁小麦产量和粮食营养品质的主要病害。研究了cv辐照（250 ~ 350 Gy）产生的春小麦M4高级突变系。Kazakhstanskaya-10研究LR抗性与空泡铁（Fe）输运之间的关系。表型和半定量组织学分析确定了高抗性突变系，表现出幼苗和成株抗性。抗性基因型表现出早期吸体前的超敏反应，这在易感野生型中是不存在的。对TaVIT4-D空泡铁转运蛋白位点（XM_044518495.1）的基因靶向序列分析显示，该区域多态性丰富，其特征是转移和翻转事件频率升高。跨膜拓扑预测和亲水分析表明，在TMH2和TMH3之间的预测胞质铁结合区域内，一些非同义的、改变电荷的氨基酸取代被定位，改变了局部的物理化学性质，同时保留了整体的跨膜结构。这些突变与籽粒铁积累增加有关，抗性最强的突变系籽粒铁浓度比野生型增加了2.11倍。总的来说，这些发现支持了TaVIT4-D变异在协调LR抗性和谷物铁积累中的潜在作用，并且与含铁营养免疫和氧化防御的模型一致。结果表明，诱导诱变影响tavit4 - d可能是提高小麦抗病性和籽粒微量营养素含量的一种有前途的非转基因策略。补充信息：在线版本包含补充资料，可在https://doi.org/10.1007/s12298-026-01812-5上获得。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.