High foliar tolerance to isoxaflutole in genetically diverse Eucalyptus urograndis progenies

IF 3.3 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2027-02-01 Epub Date: 2026-10-15 DOI:10.1016/j.cropro.2026.107906

Tharcísio Trindade Oliveira, Danilo Carvalho Pereira da Silva, Edivaldo Domingues Velini, Caio Antonio Carbonari, Magali Ribeiro da Silva, Evandro Vagner Tambarussi

{"title":"High foliar tolerance to isoxaflutole in genetically diverse Eucalyptus urograndis progenies","authors":"Tharcísio Trindade Oliveira, Danilo Carvalho Pereira da Silva, Edivaldo Domingues Velini, Caio Antonio Carbonari, Magali Ribeiro da Silva, Evandro Vagner Tambarussi","doi":"10.1016/j.cropro.2026.107906","DOIUrl":null,"url":null,"abstract":"<div><div>Herbicide selectivity in forest species remains poorly understood, particularly for HPPD inhibitors. We evaluated foliar isoxaflutole tolerance in open-pollinated <em>Eucalyptus</em> “urograndis” progenies (<em>Eucalyptus grandis</em> x <em>Eucalyptus urophylla</em>) from three commercial clones (I-144, IPB1, and SUZA217), integrating LC–MS/MS profiling, a dose-gradient assay, and microsatellite analysis. Isoxaflutole, diketonitrile (DKN), and 2-methylsulfonyl-4-trifluoromethylbenzoic acid (BA) levels were measured over 168 h. Total recovered isoxaflutole declined after 6 h, while the internal parent compound stayed stable; among-progeny differences in BA were not significant after false-discovery-rate correction. Shoot biomass showed no consistent dose-dependent decline up to 32× the standard dose. Injury severity differed among the progenies (p = 0.0007) with the progeny × dose interaction being significant (p = 0.027); I-144 showed the greatest injury and diverged from IPB1 and SUZA217 as the dose increased. Maternal-compatibility analysis of 21 microsatellite loci showed that the 1-km isolation radius did not prevent external pollen flow. Here, all the IPB1 and SUZA217 seedlings and at least 4 out of 10 I-144 seedlings carried non-maternal alleles, with 3.67 to 6.71 alleles per locus and outcrossing rates from 0.40 to 1.00. Foliar isoxaflutole caused progeny-specific injury but no mortality or reproducible biomass loss up to 32 times the operational rate, which supports its use in eucalypt plantations.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"212 ","pages":"Article 107906"},"PeriodicalIF":3.3000,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0261219426003728","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/10/15 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Herbicide selectivity in forest species remains poorly understood, particularly for HPPD inhibitors. We evaluated foliar isoxaflutole tolerance in open-pollinated Eucalyptus “urograndis” progenies (Eucalyptus grandis x Eucalyptus urophylla) from three commercial clones (I-144, IPB1, and SUZA217), integrating LC–MS/MS profiling, a dose-gradient assay, and microsatellite analysis. Isoxaflutole, diketonitrile (DKN), and 2-methylsulfonyl-4-trifluoromethylbenzoic acid (BA) levels were measured over 168 h. Total recovered isoxaflutole declined after 6 h, while the internal parent compound stayed stable; among-progeny differences in BA were not significant after false-discovery-rate correction. Shoot biomass showed no consistent dose-dependent decline up to 32× the standard dose. Injury severity differed among the progenies (p = 0.0007) with the progeny × dose interaction being significant (p = 0.027); I-144 showed the greatest injury and diverged from IPB1 and SUZA217 as the dose increased. Maternal-compatibility analysis of 21 microsatellite loci showed that the 1-km isolation radius did not prevent external pollen flow. Here, all the IPB1 and SUZA217 seedlings and at least 4 out of 10 I-144 seedlings carried non-maternal alleles, with 3.67 to 6.71 alleles per locus and outcrossing rates from 0.40 to 1.00. Foliar isoxaflutole caused progeny-specific injury but no mortality or reproducible biomass loss up to 32 times the operational rate, which supports its use in eucalypt plantations.

查看原文本刊更多论文

遗传多样性尤大桉后代叶片对异草氟唑的高耐受性

森林物种的除草剂选择性仍然知之甚少，特别是对HPPD抑制剂。我们利用LC-MS /MS分析、剂量梯度分析和微卫星分析，评估了来自三个商业无性系（I-144、IPB1和SUZA217）的开放式授粉桉树（Eucalyptus grandis x Eucalyptus urophylla）后代叶片的异草氟唑耐受性。在168 h以上测量异草氟酮、二酮腈（DKN）和2-甲基磺酰基-4-三氟甲基苯甲酸（BA）水平。6 h后，异草氟酮的总回收率下降，而内部母体化合物保持稳定；错误发现率校正后，后代间BA差异不显著。在标准剂量的32倍以上，茎部生物量没有出现一致的剂量依赖性下降。各子代间损伤严重程度存在差异（p = 0.0007），且子代 × 剂量交互作用显著（p = 0.027）；随着剂量的增加，I-144损伤最大，与IPB1和SUZA217分化。21个微卫星位点的亲本相容性分析表明，1 km的隔离半径并没有阻止外来花粉的流动。IPB1和SUZA217幼苗全部携带非母系等位基因，I-144幼苗10株中至少4株携带非母系等位基因，每个位点携带3.67 ~ 6.71个等位基因，异交率为0.40 ~ 1.00。叶面异草氟唑可引起子代特异性伤害，但无死亡率或可再生生物量损失高达作业率的32倍，这支持其在桉树人工林中的使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.