Universal single-step warming of vitrified blastocysts: feasibility across two commercial kits and comparison with the conventional warming procedure.

IF 1.5 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of gynecology obstetrics and human reproduction Pub Date : 2026-10-16 DOI:10.1016/j.jogoh.2026.103304

Yingzhuo Gao, Lodovico Parmegiani, Gabor Vajta, Da Li

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引用次数: 0

Abstract

Conventional vitrification systems for human gametes and embryos typically necessitate brand-specific warming solutions. This retrospective longitudinal cohort study on 2,563 vitrified/warmed blastocysts investigates the efficacy of a universal single-step warming (USSW) protocol for human blastocysts vitrified using distinct commercial systems, combining vitrification and warming kits from two manufacturers: Kitazato (Japan) and VitaVitro Biotech (China) according two different warming protocols: the conventional protocol (UW) or with the single-step warming (USSW). Cryo-survival was 100% across all groups. Clinical and ongoing pregnancy, miscarriage, and live birth rates were comparable among groups. Implantation rate differed overall (p = 0.03), but no pairwise differences remained significant after Bonferroni correction. This study provides preliminary evidence suggesting that the universal single-step warming (USSW) protocol may simplify kit interchangeability and shorten the warming procedure.

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玻璃化囊胚的通用单步加热：跨两个商业试剂盒的可行性和与传统加热程序的比较。

人类配子和胚胎的传统玻璃化系统通常需要特定品牌的加热解决方案。这项对2563个玻璃化/加热囊胚的回顾性纵向队列研究调查了通用单步加热（USSW）方案对使用不同商业系统玻璃化的人类囊胚的效果，该方案结合了来自两家制造商（日本Kitazato和VitaVitro Biotech（中国））的玻璃化和加热试剂盒，根据两种不同的加热方案：常规方案（UW）或单步加热（USSW）。所有组的低温存活率均为100%。临床和持续妊娠、流产和活产率在组间具有可比性。植入率总体差异（p = 0.03），但经Bonferroni校正后，两两差异无显著性。本研究提供了初步证据，表明通用单步增温（USSW）协议可以简化试剂盒的互换性并缩短增温过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of gynecology obstetrics and human reproduction Medicine-Obstetrics and Gynecology

CiteScore

3.70

自引率

5.30%

发文量

210

审稿时长

31 days

期刊介绍： Formerly known as Journal de Gynécologie Obstétrique et Biologie de la Reproduction, Journal of Gynecology Obstetrics and Human Reproduction is the official Academic publication of the French College of Obstetricians and Gynecologists (Collège National des Gynécologues et Obstétriciens Français / CNGOF). J Gynecol Obstet Hum Reprod publishes monthly, in English, research papers and techniques in the fields of Gynecology, Obstetrics, Neonatology and Human Reproduction: (guest) editorials, original articles, reviews, updates, technical notes, case reports, letters to the editor and guidelines. Original works include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses.