The lysis mechanism of the haloarchaeal virus HFTV1.

IF 7.7 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2026-10-16 DOI:10.1016/j.celrep.2026.118117

Bastiaan P Kuiper, Marco P A van Tilburg, Leonard E Bäcker, Sourav Maity, Yogy S Ariyanto, Joshua G Petters, Zaloa Aguirre-Sourrouille, Jeroen G Nijland, Harma Karsens, Greetje A Berrelkamp-Lahpor, Colin Tittes, Johanna Wiechert, Julia Frunzke, Bertram Daum, Wouter H Roos, Siewert-J Marrink, Tessa E F Quax

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引用次数: 0

Abstract

Tailed bacteriophages can lyse their host with help of the holin-endolysin system. However, for archaeal tailed viruses (arTVs), whose archaeal hosts have a fundamentally different cell envelope, lysis mechanisms are unknown. In this study, we use the model archaeal virus-host system: Haloferax tailed virus 1 (HFTV1), which infects the methanobacteriote Haloferax gibbonsii LR2-5. We identified and characterized the lysis protein GP33, a small membrane protein that has no sequence homology with bacterial holins. Overexpression resulted in cell lysis, and the protein forms foci near the cell envelope. Molecular dynamics simulation suggests that it forms pores from which the lipids are repelled, which might result in cell lysis. Indeed, atomic force microscopy (AFM) showed that GP33 forms pores in membranes, which lead to complete membrane disruption at higher concentrations. With this work, we gain insight into a lysis mechanism from a tailed archaeal virus at the molecular level.

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盐古细菌病毒HFTV1的裂解机制。

尾状噬菌体可以在holin-endolysin系统的帮助下溶解它们的宿主。然而，对于古细菌尾部病毒（arTVs），其古细菌宿主具有根本不同的细胞包膜，裂解机制尚不清楚。在这项研究中，我们使用了模型古细菌病毒-宿主系统：盐腐尾病毒1 (HFTV1)，它感染了甲烷杆菌长臂猿盐腐尾病毒LR2-5。我们鉴定并鉴定了裂解蛋白GP33，这是一种与细菌holins没有序列同源性的小膜蛋白。过表达导致细胞裂解，蛋白在细胞包膜附近形成灶。分子动力学模拟表明，它形成了脂质被排斥的孔，这可能导致细胞裂解。事实上，原子力显微镜（AFM）显示GP33在膜上形成孔隙，在较高浓度下导致完全的膜破坏。通过这项工作，我们在分子水平上深入了解了尾古菌病毒的裂解机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.