Cross-kingdom RNA interference-mediated silencing of SsStuA to control Sclerotinia sclerotiorum in Brassica napus

IF 4.4 Q3 Agricultural and Biological Sciences

Oil Crop Science Pub Date : 2026-09-01 Epub Date: 2026-09-11 DOI:10.1016/j.ocsci.2026.05.007

Xijin Huang, Lili Liu, Yanlun Li, Xixi Wei, Shuxiang Mao, Rongkui Hui, Wenqing Tan, Lei Qin, Mingli Yan

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Abstract

Sclerotinia stem rot, caused by the fungal pathogen Sclerotinia sclerotiorum, inflicts substantial economic losses on rapeseed production. The lack of resistant germplasm has rendered disease management heavily reliant on chemical fungicides, and prolonged application of these fungicides often leads to the development of fungicide resistance. Consequently, there is an imperative need for novel antifungal strategies. In this study, we identified the virulence-associated factor SsStuA via mutagenesis screening. Host-induced gene silencing (HIGS) targeting SsStuA significantly enhanced the resistance of Nicotiana benthamiana to S. sclerotiorum. Furthermore, spray-induced gene silencing (SIGS) directed at SsStuA effectively suppressed infections caused by both S. sclerotiorum and Botrytis cinerea on rapeseed leaves. These findings demonstrate that SsStuA serves as a promising target for cross-kingdom RNA interference (RNAi)-based fungal control approaches, including HIGS and SIGS.

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跨界RNA干扰介导SsStuA沉默控制甘蓝型油菜菌核病

油菜菌核病是由菌核菌引起的一种病害，对油菜生产造成严重的经济损失。抗性种质的缺乏使得疾病管理严重依赖化学杀菌剂，而这些杀菌剂的长期使用往往导致杀菌剂耐药性的发展。因此，迫切需要新的抗真菌策略。在这项研究中，我们通过诱变筛选确定了毒力相关因子SsStuA。针对SsStuA的宿主诱导基因沉默（HIGS）显著增强了本烟对菌核病菌的抗性。此外，针对SsStuA的喷雾诱导基因沉默（SIGS）可以有效抑制菌核葡萄球菌和灰霉病菌对油菜叶片的感染。这些发现表明，SsStuA是基于跨界RNA干扰（RNAi）的真菌控制方法（包括HIGS和SIGS）的有希望的靶标。

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