Zhi-Long Liu, Qian Zhang, Qin Lu, Yan-Jing Zhang, Li Li, Yu-Fang Zhao, Guo-Lin Yin, Xiao-Hui Yan, Yan-Hua Zhang, Li-Ping Liu
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引用次数: 0
Abstract
Objective: The aim of this study was to develop a rabbit model of inflammatory pseudotumor of the liver (IPTL) using an ultrasound-guided implantation technique and to assess its feasibility, safety, and imaging characteristics in comparison with a surgical embedding approach.
Methods: Twenty-six New Zealand rabbits were randomly assigned to one of three groups: the ultrasound-guided implantation group (Experimental Group), the surgical embedding group (Surgical Group), or a blank control group. IPTL lesions were induced using a combination of gelatin sponge and Freund's complete adjuvant. Parameters assessed included operative duration, lesion modeling success rate, findings from conventional ultrasound and contrast-enhanced ultrasound (CEUS), laboratory indicators, and histopathological outcomes.
Results: All animals survived without severe complications. The lesion modeling success rates were 90% in the Experimental Group and 95% in the Surgical Group (p > 0.05), while operative duration was significantly reduced in the Experimental Group (p < 0.05). On conventional ultrasound, IPTL lesions in both experimental groups presented as hyperechoic nodules with well-defined margins. CEUS indicated three distinct enhancement patterns: isoenhancement, hypoenhancement, and rapid washout. Laboratory testing demonstrated no significant differences in serum hyaluronic acid or liver function markers between the Experimental and control groups; however, the Surgical Group exhibited significantly elevated hyaluronic acid levels (p < 0.05). Immunohistochemical analysis demonstrated lower microvessel density in hypoenhanced nodules compared to those with other enhancement patterns.
Conclusion: The ultrasound-guided implantation technique is a safe, efficient, and reproducible method for modeling IPTL in rabbits. The resulting lesions exhibit histopathological and CEUS characteristics that closely resemble those observed in human IPTL, supporting its utility as an experimental platform for further research into the pathophysiology and imaging features of IPTL.
期刊介绍：
Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy.
Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field.
Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.
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