A Derived Moving Reaction Boundary Initially Created by Formic Buffer and Sodium Hydroxide in Capillary Electrophoresis: Theoretic and Experimental Study.

IF 2.6 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2026-10-15 DOI:10.1002/elps.70168

Jing Gao, Weiwen Liu, Qiang Zhang, Chengxi Cao

引用次数: 0

Abstract

In this article, we investigate a derived moving reaction boundary (MRB) generated upon current application in a discontinuous electrolyte system consisting of formic buffer (anolyte) and sodium hydroxide (catholyte). The model showcases the formations of two derived zones, including an acidic zone (Zone β1) and an alkaline zone (Zone β2), and the subsequent development of a derived MRB between Zones β1 and β2. The proposed model was validated through numerical simulations and experiments using capillary electrophoresis and gel-based visualization, with insulin and phenylalanine selected as model analytes. Quantitative comparisons demonstrate good agreement between the derived MRB theory and experimental observations, whereas the conventional model fails to accurately predict the observed boundary behavior. This work provides new insights into reaction boundary dynamics in weak acid-strong base electrolyte systems and offers practical guidance for sample stacking strategies in peptide and protein electrophoresis.

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甲酸缓冲液和氢氧化钠在毛细管电泳中初步形成的移动反应边界：理论和实验研究。

在本文中，我们研究了在由甲酸缓冲液（阳极液）和氢氧化钠（阴极液）组成的不连续电解质系统中电流应用产生的派生移动反应边界（MRB）。该模型展示了两个衍生带的形成，包括酸性带（β1区）和碱性带（β2区），以及随后在β1和β2区之间衍生的MRB的发展。以胰岛素和苯丙氨酸为模型分析物，采用毛细管电泳和凝胶可视化技术对模型进行了数值模拟和实验验证。定量比较表明，推导的MRB理论与实验观测结果吻合良好，而传统模型无法准确预测观察到的边界行为。这项工作为弱酸-强碱电解质系统的反应边界动力学提供了新的见解，并为肽和蛋白质电泳的样品堆积策略提供了实用的指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.