[Bioactivity of multi-matrix bioceramic root canal sealer: An in vitro study].

Q3 Medicine

北京大学学报（医学版） Pub Date : 2026-10-18

Weijia Lyu, Wei Bai, Xiaoyan Wang, Yuhong Leung

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引用次数: 0

Abstract

Objective: To investigate in vitro bioactivity of the bioceramic root canal sealer Bioseal.

Methods: An in vitro mineralization model consisting of root canal sealer specimens and simulated body fluid (SBF) was constructed. Bioceramic-based root canal sealers Bioseal, iRoot SP, and silicone root canal sealer GuttaFlow2 were each prepared into cylindrical specimens with a diameter of 10 mm and a height of 2 mm. All the root canal sealer specimens were then immersed in SBF and maintained at 37 ℃ in a water bath for 4 weeks. Scanning electron microscopy (SEM) and X-ray energy dispersive spectroscopy (EDS) were used to observe the morphology of the root canal sealer specimens after immersion. The surface composition was analyzed by X-ray diffraction (XRD). Calcium ion concentration in the extraction solution was measured using inductively coupled plasma optical emission spectrometry (ICP-OES) and pH value was monitored for 1, 2, 7, 14, 21, and 28 days. Statistical analysis was performed using one-way ANOVA test. The level of significance was set at P < 0.05.

Results: After 4 weeks of immersion in SBF, spherical particles were observed on the surfaces of both Bioseal and iRoot SP under SEM. XRD analysis confirmed that the calcium-to-phosphorus ratios were 1.64 and 1.56, respectively, indicating the formation of hydroxyapatite. The calcium ion concentration in bioceramic-based root canal sealers Bioseal and iRoot SP extraction solutions peaked at (110.4±2.8) mg/L and (125.4±3.8) mg/L after 7 days, respectively (P < 0.05), then stabilized (P>0.05). After 2 days, the pH value peaked at 7.99±0.11 and 8.23±0.10, respectively (P < 0.05). The calcium ion concentration and pH value of Bioseal extraction solution were lower than those of iRoot SP at all time points (P < 0.05). No hydroxyapatite formation was observed on the sample surface of silicone root canal sealer GuttaFlow2, and during the evaluation period of 28 days, there were no significant changes in calcium ion concentration or pH value (P>0.05).

Conclusion: The bioceramic root canal sealer Bioseal and iRoot SP demonstrated bioactivity by promoting calcium ion deposition and forming hydroxyapatite in physiological solutions.

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多基质生物陶瓷根管封闭剂生物活性的体外研究

目的：研究生物陶瓷根管密封材料Bioseal的体外生物活性。方法：建立由根管封闭器标本和模拟体液组成的体外矿化模型。生物陶瓷根管密封剂Bioseal、iRoot SP和硅胶根管密封剂GuttaFlow2分别制备成直径为10 mm、高度为2 mm的圆柱形标本。所有根管封闭器标本浸泡在SBF中，在37℃水浴中保存4周。采用扫描电镜（SEM）和x射线能谱仪（EDS）观察浸泡后根管封闭器标本的形态。用x射线衍射（XRD）分析了其表面成分。采用电感耦合等离子体发射光谱法（ICP-OES）测定萃取液中的钙离子浓度，并监测pH值，持续1、2、7、14、21和28 d。统计学分析采用单因素方差分析。P < 0.05为显著性水平。结果：在SBF中浸泡4周后，在扫描电镜下，Bioseal和iRoot SP表面均可见球形颗粒。XRD分析证实，钙磷比分别为1.64和1.56，表明形成了羟基磷灰石。生物陶瓷根管密封剂Bioseal和iRoot SP提取液中钙离子浓度在7 d后分别达到峰值（110.4±2.8）mg/L和（125.4±3.8）mg/L (P < 0.05)，随后趋于稳定（P < 0.05）。2 d后pH值最高，分别为7.99±0.11和8.23±0.10 （P < 0.05）。各时间点Bioseal提取液的钙离子浓度和pH值均低于iRoot SP （P < 0.05）。硅胶根管封口剂GuttaFlow2样品表面未见羟基磷灰石形成，在28天的评价期内，钙离子浓度和pH值无显著变化（P < 0.05）。结论：生物陶瓷根管封闭剂Bioseal和iRoot SP在生理溶液中促进钙离子沉积，形成羟基磷灰石，具有生物活性。

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来源期刊

北京大学学报（医学版） Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

9815

期刊介绍： Beijing Da Xue Xue Bao Yi Xue Ban / Journal of Peking University (Health Sciences), established in 1959, is a national academic journal sponsored by Peking University, and its former name is Journal of Beijing Medical University. The coverage of the Journal includes basic medical sciences, clinical medicine, oral medicine, surgery, public health and epidemiology, pharmacology and pharmacy. Over the last few years, the Journal has published articles and reports covering major topics in the different special issues (e.g. research on disease genome, theory of drug withdrawal, mechanism and prevention of cardiovascular and cerebrovascular diseases, stomatology, orthopaedic, public health, urology and reproductive medicine). All the topics involve latest advances in medical sciences, hot topics in specific specialties, and prevention and treatment of major diseases. The Journal has been indexed and abstracted by PubMed Central (PMC), MEDLINE/PubMed, EBSCO, Embase, Scopus, Chemical Abstracts (CA), Western Pacific Region Index Medicus (WPR), JSTChina, and almost all the Chinese sciences and technical index systems, including Chinese Science and Technology Paper Citation Database (CSTPCD), Chinese Science Citation Database (CSCD), China BioMedical Bibliographic Database (CBM), CMCI, Chinese Biological Abstracts, China National Academic Magazine Data-Base (CNKI), Wanfang Data (ChinaInfo), etc.