Capillary-integrated chiral gold nanocrystals for label-free SERS detection of serum amyloid-β 1–42 in Alzheimer’s disease rats

IF 5.4 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2026-10-16 DOI:10.1007/s00604-026-08457-x

Yangfan Zhou, Huaiwu Fu, Kaiyong Chen, Yixuan Jiang, Zixun Wang, Weixing Wu, Xiaowei Cao

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Abstract

Chiral plasmonics was integrated with surface-enhanced Raman scattering (SERS) technology to develop a high-performance chiral SERS sensor with promising applications for the early identification and ultrasensitive detection of Alzheimer’s disease. The sensor utilizes the self-assembly of chiral gold nanocrystals (CG NCs) on the inner wall of a capillary, introducing a surface-enhanced Raman scattering chiral anisotropy (SERS-ChA) effect. Consequently, a label-free chiral SERS sensor ‌is‌ constructed. In the presence of the target analyte Amyloid-β 1–42 (Aβ₁₋₄₂, Aβ₄₂), CG NCs generate an electric-dipole-magnetic-dipole coupling effect within the chiral plasmonic field, resulting from selective resonance coupling between enantiomer-induced electric and magnetic dipole (MD) moments. This process significantly enhances the chiral asymmetric response and amplifies the SERS signal, allowing highly sensitive recognition and detection of Aβ₄₂. The sensor exhibits excellent analytical performance, achieving a limit of detection (LOD) as low as 0.0899 pg/mL. Furthermore, it demonstrates strong selectivity for Aβ₄₂ and robust resistance to interference from other substances commonly present in serum. By analyzing serum samples from rat models of Alzheimer’s disease and comparing the results with those from an Aβ1–42 ELISA kit, a high correlation (R² = 0.976) was demonstrated, validating the sensor’s practicality and accuracy and indicating its stable and reliable quantitative performance in complex biological matrices. This study provides new insights and theoretical foundations for the early screening and diagnosis of Alzheimer’s disease.

Graphical Abstract

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毛细管集成手性金纳米晶体用于阿尔茨海默病大鼠血清淀粉样蛋白-β 1-42无标记SERS检测

将手性等离子体与表面增强拉曼散射（SERS）技术相结合，开发了一种高性能的手性SERS传感器，在阿尔茨海默病的早期识别和超灵敏检测中具有广阔的应用前景。该传感器利用手性金纳米晶体（CG NCs）在毛细管内壁的自组装，引入了表面增强拉曼散射手性各向异性（SERS-ChA）效应。因此，构建了一个无标记的手性SERS传感器。当目标分析物Amyloid-β 1-42 （Aβ1−42,Aβ42）存在时，CG nc在手性等离子体场中产生电偶极子-磁偶极子-偶极子耦合效应，这是由对映体诱导的电偶极子和磁偶极子（MD）矩之间的选择性共振耦合造成的。这一过程显著增强了手性不对称反应，放大了SERS信号，实现了a - β42的高灵敏度识别和检测。该传感器具有优异的分析性能，检测限（LOD）低至0.0899 pg/mL。此外，它对Aβ42具有很强的选择性，并且对血清中常见的其他物质的干扰具有很强的抵抗力。通过对阿尔茨海默病大鼠模型的血清样本进行分析，并与a β1 - 42 ELISA试剂盒的结果进行比较，结果显示高相关性（R2 = 0.976），验证了传感器的实用性和准确性，表明其在复杂生物基质中的定量性能稳定可靠。本研究为阿尔茨海默病的早期筛查和诊断提供了新的见解和理论基础。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.