Complete genome sequencing and molecular characterization of Jasminum polyanthum rubodvirus 1, a virus representative of a putative novel species in the genus Rubodvirus

Abstract

To identify viruses infecting Jasminum polyanthum (pink jasmine), symptomatic leaves were collected from Yunnan Province, China, and subjected to virus detection and molecular characterization. A virus was identified and tentatively named Jasminum polyanthum rubodvirus 1 (JPRV1). The virus belongs to the genus Rubodvirus within the family Phenuiviridae (order Hareavirales). The genome of JPRV1 consists of three negative-sense single-stranded RNA segments: RNA1 (7,204 nt), RNA2 (1,492 nt), and RNA3 (1,466 nt), encoding the RNA-dependent RNA polymerase (RdRp; L protein), movement protein (MP), and nucleocapsid protein (N), respectively. Sequence comparisons showed that the JPRV1 L protein shared 37.46%–42.87% amino acid sequence identity with those of recognized Rubodvirus species. Phylogenetic analysis placed JPRV1 in a distinct lineage within the genus Rubodvirus. These findings support the classification of JPRV1 as a virus representative of a putative novel species in the genus Rubodvirus and provide the first report of a rubodvirus associated with J. polyanthum.