Development of Sustainable Bioactive Glass-Ceramic/Gelatin/Collagen/Carboxymethyl Cellulose Biomedical Dressings via Tape Casting

IF 5.6 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2026-10-15 DOI:10.1007/s10924-026-03996-z

Maria Eduarda Xavier da Silva, Isabela Karina Della-Flora, Karina Cesca, Patrícia Bulegon Brondani, Luciano Senff, Ana Paula Serafini Immich, Dachamir Hotza

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引用次数: 0

Abstract

Chronic wounds require dressings that actively manage moisture, control infection, and support tissue repair, creating a demand for sustainable and effective scaffolds manufacturing. Although bioactive glass-based composites and gelatin/collagen systems have shown promise for biomaterials manufacturing, the development of bioactive dressings for wound care by tape casting remain underexplored. In this research, tape casting is used to develop bioactive and biodegradable dressings by incorporating bioactive glass (BG) -ceramic microparticles. Gelatin (GEL), collagen (COL), and carboxymethyl cellulose (CMC) are used as sustainable additives in the tape casting process, and genipin (GNP) as a natural crosslinking agent to replace the sintering process. The BG-ceramic suspensions are optimized using a Design of Experiments (DoE) with GEL and COL concentrations of 0, 3.25, 5, 6.5, and 10% (w/w). Among the tested formulations, a gelatin-based tape (BG-100) cast at 0.08 m/s produced porous, continuous, and easily handled dressings with a thickness of 0.5 ± 0.2 mm. Then, the dried BG-100 samples were crosslinked with genipin (BG-100-GNP). Genipin crosslinking increased toughness from 2.29 to 4.96 J/m³, elongation at break from 0.95 to 3.15%, and the contact angle decreased from 61.53 ± 2.79° to 0°, consistent with improved wettability relevant to wound contact. The crosslinked dressing was non-cytotoxic to L929 fibroblasts in indirect assay, inhibited Staphylococcus aureus growth in 24 h, and showed about 60% mass loss over 7 weeks. These results demonstrate that genipin-crosslinked bioactive glass-ceramic-based dressings produced by tape casting represent a rapid, sustainable, and promising platform for further support soft tissue regeneration and wound healing.

Graphical Abstract

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可持续生物活性玻璃陶瓷/明胶/胶原蛋白/羧甲基纤维素生物医用敷料的开发

慢性伤口需要积极控制水分、控制感染和支持组织修复的敷料，这就产生了对可持续和有效的支架制造的需求。尽管生物活性玻璃基复合材料和明胶/胶原蛋白系统在生物材料制造方面已经显示出前景，但用于伤口护理的生物活性敷料的开发仍未得到充分探索。在这项研究中，胶带铸造被用于开发生物活性和可生物降解敷料，通过加入生物活性玻璃(BG) -陶瓷微粒。明胶（GEL）、胶原蛋白（COL）和羧甲基纤维素（CMC）被用作胶带浇注过程中的可持续添加剂，genipin （GNP）被用作天然交联剂来取代烧结过程。使用实验设计（DoE）对bg -陶瓷悬浮液进行优化，GEL和COL浓度分别为0、3.25、5、6.5和10% （w/w）。在测试的配方中，以0.08 m/s的速度浇注的明胶基胶带（BG-100）产生了多孔、连续且易于处理的敷料，厚度为0.5±0.2 mm。然后，干燥后的BG-100样品与genipin （BG-100- gnp）交联。Genipin交联将韧性从2.29提高到4.96 J/m³，断裂伸长率从0.95提高到3.15%，接触角从61.53±2.79°降低到0°，与伤口接触相关的润湿性改善一致。间接实验表明，交联敷料对L929成纤维细胞无细胞毒性，24 h内抑制金黄色葡萄球菌生长，7周内质量下降约60%。这些结果表明，通过胶带铸造生产的genipin交联生物活性玻璃陶瓷基敷料代表了一个快速、可持续和有前途的平台，可以进一步支持软组织再生和伤口愈合。图形抽象

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.