Nine minutes is enough: An accelerated fMRI protocol for reliable connectivity mapping in healthy young adults.

Abstract

Introduction: Resting‑state fMRI is widely used to map brain connectivity, yet the field lacks a clear, data‑driven standard for acquisition duration. We compared traditional Single‑Band (SB) imaging with an accelerated Multiband factor 4 (MB4) protocol to identify a time‑efficient approach for capturing stable network topology.

Methods: Twenty‑five healthy young adults completed two sessions one month apart, each including counterbalanced 13‑minute SB and MB4 scans. Test-retest reliability was evaluated across multiple durations using Portrait Divergence, a multiscale graph‑theoretic metric. Supplementary analyses compared Portrait Divergence with the field-standard Intraclass Correlation Coefficient, assessed robustness across preprocessing pipelines and parcellation schemes, and examined MB4's ability to capture both cortical and subcortical connectivity.

Results: MB4 consistently outperformed SB across all tested durations, with peak topological stability at 9 minutes. Stability declined beyond this point, likely due to cumulative motion artefacts. Supplementary analyses confirmed MB4's robustness across preprocessing strategies and atlas choices and demonstrated effective whole‑brain coverage.

Conclusions: In healthy young adults, MB4 captures network topology more effectively than SB and reaches peak stability at 9 minutes before declining, challenging the assumption that longer scans are always better. These findings require validation in clinical, paediatric, and geriatric cohorts.