Association of miR-130a-5p rs731384 With the Susceptibility of Peri-Implantitis and the Function of miR-130a-5p in Peri-Implantitis.

Abstract

Peri-implantitis (PI) is a common inflammatory disease that threatens the stability of dental implants. This study investigated whether the promoter polymorphism rs731384 of the miR-130a gene is linked to PI susceptibility and further explored the functional role of miR-130a-5p in inflammatory responses and osteoblast viability. A total of 143 patients without PI and 127 patients with PI were gathered in this study. MassARRAY was used to genotype rs731384. Saliva samples were collected to measure miR-130a-5p expression (via reverse transcriptase-polymerase chain reaction [RT-PCR]) and inflammatory cytokine levels (including interleukin [IL]-1β, IL-6 and tumour necrosis factor [TNF]-α, via enzyme-linked immunosorbent assay [ELISA]). In vitro inflammation model was established by treating hFOB1.19 human osteoblasts with lipopolysaccharide (LPS). The functional role of miR-130a-5p was assessed by transfecting cells with a miR-130a-5p inhibitor and evaluating its effects on cell viability, apoptosis and cytokine release in the supernatants. Bioinformatic databases (miRDB, ENCORI and TargetScan) were utilized to predict downstream target genes and pathways. A Positive association has been discovered between rs731384 and PI susceptibility under GA versus GG (odds ratio [OR] = 1.758, 95% confidence interval [CI] = 1.017-3.036), A versus G (OR = 1.779, 95% CI = 1.140-2.778), and GA + AA versus GG (OR = 1.867, 95% CI = 1.110-3.140) models. Upregulated miR-130a-5p showed promising diagnostic value for distinguishing PI from healthy controls (area under the curve [AUC] = 0.858, sensitivity = 80.31%, specificity = 79.02%). Spearman analysis revealed a positive correlation between miR-130a-5p and periodontal parameters as well as inflammation factors (IL-1β, IL-6, TNF-α and C-reactive protein [CRP]). The miR-130a inhibitor reversed the LPS-induced reduction in cell viability, the increase of apoptosis and the enhancement of IL-1β, IL-6 and TNF-α concentrations. Bioinformatics predicted that miR-130a-5p targets genes enriched in TGF-β, Hippo and FoxO signalling pathways. The mir-130a-5p rs731384 polymorphism is positively correlated with PI susceptibility. High miR-130a-5p may contribute to the diagnosis of PI from healthy controls. The inflammation and metabolism of bone tissue in PI may be significantly influenced by miR-130a.