Profile and classification of invasive fungal disease in patients with haematologic malignancies using microscopy, culture, biomarkers and polymerase chain reaction.

IF 1.5 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology Pub Date : 2026-10-14 DOI:10.1016/j.ijmmb.2026.101319

Moumita Chakraborty, Malini R Capoor, Sheetal Sharma, Sumita Chaudhary, Rupi Jamwal

{"title":"Profile and classification of invasive fungal disease in patients with haematologic malignancies using microscopy, culture, biomarkers and polymerase chain reaction.","authors":"Moumita Chakraborty, Malini R Capoor, Sheetal Sharma, Sumita Chaudhary, Rupi Jamwal","doi":"10.1016/j.ijmmb.2026.101319","DOIUrl":null,"url":null,"abstract":"Background: Invasive fungal diseases (IFDs) are significant causes of morbidity and mortality in patients with haematologic malignancies (HM). This study aimed to classify IFD using integrated diagnostic methods according to European Organization for Research and Treatment of Cancer/Mycoses Study Group Education and Research Consortium (EORTC/MSGERC) criteria.Methods: A prospective observational study was conducted in 100 adult HM patients not on antifungal prophylaxis. Diagnosis included microscopy, culture, galactomannan (GM) enzyme-linked immunosorbent assay (ELISA), Candida mannan immunoglobulin M (IgM) lateral flow assay (LFA), Aspergillus IgM assay, cryptococcal antigen LFA, (1,3)-β-D-glucan (BDG) assay and panfungal polymerase chain reaction (PCR), along with high-resolution computed tomography (HRCT).Results: Probable IFD was observed in 51%, possible in 24%, proven in 12% and no IFD in 13%. Acute leukaemia predominated, with invasive aspergillosis (IA) and candidiasis being the most common infections. Fever was the most frequent presentation, while respiratory symptoms were associated with invasive aspergillosis. PCR showed the highest sensitivity (88.8%), followed by BDG (87.3%). Microscopy and culture were positive in 25% cases, Aspergillus fumigatus being the most common isolate. HRCT was normal in 56%, with ground-glass opacities and consolidation as the most frequent abnormalities.Conclusion: Integrated diagnostic approaches improve classification and early detection of IFD in HM patients and support timely antifungal management.","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":" ","pages":"101319"},"PeriodicalIF":1.5000,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Medical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ijmmb.2026.101319","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Invasive fungal diseases (IFDs) are significant causes of morbidity and mortality in patients with haematologic malignancies (HM). This study aimed to classify IFD using integrated diagnostic methods according to European Organization for Research and Treatment of Cancer/Mycoses Study Group Education and Research Consortium (EORTC/MSGERC) criteria.

Methods: A prospective observational study was conducted in 100 adult HM patients not on antifungal prophylaxis. Diagnosis included microscopy, culture, galactomannan (GM) enzyme-linked immunosorbent assay (ELISA), Candida mannan immunoglobulin M (IgM) lateral flow assay (LFA), Aspergillus IgM assay, cryptococcal antigen LFA, (1,3)-β-D-glucan (BDG) assay and panfungal polymerase chain reaction (PCR), along with high-resolution computed tomography (HRCT).

Results: Probable IFD was observed in 51%, possible in 24%, proven in 12% and no IFD in 13%. Acute leukaemia predominated, with invasive aspergillosis (IA) and candidiasis being the most common infections. Fever was the most frequent presentation, while respiratory symptoms were associated with invasive aspergillosis. PCR showed the highest sensitivity (88.8%), followed by BDG (87.3%). Microscopy and culture were positive in 25% cases, Aspergillus fumigatus being the most common isolate. HRCT was normal in 56%, with ground-glass opacities and consolidation as the most frequent abnormalities.

Conclusion: Integrated diagnostic approaches improve classification and early detection of IFD in HM patients and support timely antifungal management.

查看原文本刊更多论文

概要文件和分类患者侵袭性真菌病恶性血液疾病过程使用显微镜,文化、生物标志物和聚合酶链反应。

背景：侵袭性真菌病（IFDs）是血液学恶性肿瘤（HM）患者发病和死亡的重要原因。本研究旨在根据欧洲癌症研究和治疗组织/真菌研究小组教育和研究联盟（EORTC/MSGERC）的标准，使用综合诊断方法对IFD进行分类。方法：对100例未进行抗真菌预防治疗的成年HM患者进行前瞻性观察研究。诊断包括显微镜检查、培养、半乳甘露聚糖（GM）酶联免疫吸附试验（ELISA）、甘露甘露聚糖免疫球蛋白M （IgM）侧流试验（LFA）、曲霉菌IgM试验、隐球菌抗原LFA、(1,3)-β- d -葡聚糖（BDG）试验和全真菌聚合酶链反应（PCR），以及高分辨率计算机断层扫描（HRCT）。结果：有可能的IFD占51%，可能的占24%，证实的占12%，无IFD的占13%。急性白血病占主导地位，侵袭性曲霉病（IA）和念珠菌病是最常见的感染。发烧是最常见的表现，而呼吸道症状与侵袭性曲霉病有关。PCR检测灵敏度最高（88.8%），其次为BDG（87.3%）。显微镜和培养25%的病例呈阳性，烟曲霉是最常见的分离物。HRCT正常的占56%，最常见的异常是磨玻璃混浊和实变。结论：综合诊断方法有助于HM患者IFD的分类和早期发现，并支持及时的抗真菌治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.