A Broad-Spectrum Antagonistic Bacillus Velezensis Strain HY17 as a Potential Biocontrol Agent Aagainst Major Maize Fungal Diseases.

IF 2.8 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2026-10-14 DOI:10.1007/s00284-026-05192-7

Yao Dong, Jiahe Liu, Xin Chen, Tianpeng Zhang, Xianming Zhou, Yi Han, Fuping Song, Jie Li, Xin Zhang

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Abstract

Maize fungal diseases seriously threaten crop productivity and grain quality, highlighting the need for effective and sustainable biocontrol agents. In this study, an antagonistic bacterium, HY17, was isolated from maize rhizosphere soil and identified as Bacillus velezensis based on morphological characterization and phylogenomic analysis. HY17 exhibited broad-spectrum antifungal activity against several major maize fungal pathogens. Its cell-free fermentation supernatant showed clear concentration-dependent inhibition of fungal growth, indicating that extracellular metabolites play an important role in their antagonistic activity. HY17 also displayed broad growth adaptability under different culture media, temperature, and pH conditions. Whole-genome analysis revealed that HY17 contains a chromosome of 3,931,127 bp and multiple biosynthetic gene clusters associated with antimicrobial secondary metabolites. Crude lipopeptide extracts from HY17 further exhibited concentration-dependent antifungal activity. PCR assays detected representative antimicrobial biosynthetic genes, including bacA, bmyB, ituC, fenD, and srfAA, and LC-MS analysis revealed characteristic ion peaks putatively assigned to iturin-family lipopeptides and a fengycin-family component. Collectively, these results indicate that B. velezensis HY17 is a promising biocontrol candidate for maize fungal diseases, with lipopeptide-related metabolites likely contributing substantially to its broad-spectrum antifungal activity.

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一株广谱拮抗Velezensis芽孢杆菌HY17作为玉米主要真菌病害的潜在生物防治剂

玉米真菌病害严重威胁作物产量和粮食品质，迫切需要有效和可持续的生物防治剂。本研究从玉米根际土壤中分离到一株拮抗细菌HY17，经形态鉴定和系统基因组分析鉴定为芽孢杆菌。HY17对几种主要玉米真菌病原菌具有广谱抗真菌活性。其无细胞发酵上清液对真菌生长有明显的浓度依赖性抑制作用，表明胞外代谢物在其拮抗活性中起重要作用。HY17在不同的培养基、温度和pH条件下也表现出广泛的生长适应性。全基因组分析显示，HY17含有一条3,931,127 bp的染色体和多个与抗菌次级代谢物相关的生物合成基因簇。HY17粗脂肽提取物进一步表现出浓度依赖性的抗真菌活性。PCR检测到具有代表性的抗菌生物合成基因，包括bacA、bmyB、ituC、fins和srfAA， LC-MS分析显示了iturin家族脂肽和fengycin家族成分的特征离子峰。综上所述，这些结果表明，B. velezensis HY17是一种很有前景的玉米真菌病生物防治候选者，其脂肽相关代谢物可能对其广谱抗真菌活性起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.