Microenvironment shapes Staphylococcus aureus phenotypes in children with cystic fibrosis over time.

IF 4.5 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2026-10-01 eCollection Date: 2026-10-16 DOI:10.1016/j.isci.2026.117708

Stefania A Robaldi, Rosana Pereda, Maria Constanza Pautasso, Enrique Blasko, Ailen Fretes, Trinidad Jaureguialzo, Mateo Diaz Appella, Mariana B Galeano, Maria Jose Rial, Silvina Smith, Eugenia Ginestet, Norma Gonzalez, Javier M González, Andrea Smania, Alejandro J Vila, Claudia Sola, Pablo M Cassanelli, Paula M Tribelli

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Abstract

Staphylococcus aureus is a common pathogen in children with cystic fibrosis (CF), frequently preceding or coexisting with Pseudomonas aeruginosa. The impact of transient P. aeruginosa infection and therapeutic intervention on S. aureus phenotypic shaping remains poorly understood. We characterized 546 isolates from a longitudinal cohort of sixteen pediatric patients in CF follow-up, either monoinfected or coinfected, over eighteen months. Isolates were grouped into three phenotypic clusters. Monoinfected patients were enriched in a cluster with high pigment and biofilm, whereas transient P. aeruginosa detection was associated with a shift toward reduced pigment and biofilm, while coinfected patients maintained stable phenotypes. Phenotypes were determined by the airway microenvironment rather than by clonal background. In two monoinfected patients, this shift coincided with transient infection and ciprofloxacin treatment with increased MRSA; in vitro, MRSA predominated over MSSA when both P. aeruginosa and ciprofloxacin were present, underscoring the need to monitor S. aureus phenotypic transitions.

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随着时间的推移，微环境会影响囊性纤维化儿童的金黄色葡萄球菌表型。

金黄色葡萄球菌是儿童囊性纤维化（CF）的常见病原体，常先于或与铜绿假单胞菌共存。短暂的铜绿假单胞菌感染和治疗干预对金黄色葡萄球菌表型形成的影响仍然知之甚少。我们对16例CF患儿进行了为期18个月的单感染或共感染的纵向队列随访。将分离物分为3个表型簇。单一感染的患者在高色素和生物膜的集群中富集，而短暂的铜绿假单胞菌检测与色素和生物膜的减少有关，而共同感染的患者保持稳定的表型。表型是由气道微环境而不是克隆背景决定的。在两名单感染患者中，这种转变与短暂感染和环丙沙星治疗相吻合，MRSA增加；在体外，当铜绿假单胞菌和环丙沙星同时存在时，MRSA比MSSA占优势，这强调了监测金黄色葡萄球菌表型转变的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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