Genome-wide mapping of the Galleria mellonella transcription start sites during fungal infection and treatment.

IF 4.5 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2026-10-01 eCollection Date: 2026-10-16 DOI:10.1016/j.isci.2026.117675

Imad Abugessaisa, Mickey Konings, Ri-Ichiroh Manabe, Michihira Tagami, Jessica Severin, Akira Hasegawa, Tsugumi Kawashima, Hiroko Kinoshita, Shohei Noma, Chitose Takahashi, Annelies Verbon, Yasushi Okazaki, Wendy W J van de Sande, Takeya Kasukawa

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引用次数: 0

Abstract

Galleria mellonella is an emerging experimental model, but its current genome annotation lacks transcription start site (TSS) information. Because TSS annotation is essential for understanding transcriptional regulation, we used low quantity single strand CAGE (LQ-ssCAGE) to map cap analysis of gene expression (CAGE)-defined TSSs from 72 G. mellonella larval samples. Among these, 36 larvae were infected with the fungal pathogen Madurella mycetomatis and 36 served as healthy controls, allowing identification of TSSs under both infected and healthy conditions. Infected and healthy larvae were treated with itraconazole or ravuconazole. To assess key characteristics of the CAGE data, TSS peaks and putative enhancers were intersected with the existing genome annotation for annotation and characterization. This analysis identified two sets of TSS peaks, permissive and robust, representing the first draft TSS atlas for G. mellonella. The atlas-derived annotations showed a markedly stronger core promoter sequence signature than the NCBI reference annotation, supporting improved resolution and accuracy of TSS annotation in the atlas.

查看原文本刊更多论文

真菌感染和治疗过程中mellongalleria转录起始位点的全基因组定位。

mellonella是一种新兴的实验模型，但其目前的基因组注释缺乏转录起始位点（transcription start site， TSS）信息。由于TSS注释对于理解转录调控至关重要，因此我们使用低数量单链CAGE （LQ-ssCAGE）对72份黄颡鱼幼虫样本的基因表达（CAGE）定义的TSS进行了图谱分析。其中36只幼虫感染真菌病原体足跖Madurella mycetomatis， 36只作为健康对照，在感染和健康条件下均可鉴定出tss。感染幼虫和健康幼虫分别用伊曲康唑或拉武康唑处理。为了评估CAGE数据的关键特征，将TSS峰和假定的增强子与现有的基因组注释相交，以进行注释和表征。该分析鉴定出两组TSS峰，允许峰和稳健峰，代表了mellonella的TSS图谱初稿。该图谱衍生的注释比NCBI参考注释显示出更强的核心启动子序列特征，支持了图谱中TSS注释的分辨率和准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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