Chaperone protein DnaK-mediated regulation of spore coat assembly in Bacillus subtilis.

IF 4.5 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2026-09-30 eCollection Date: 2026-10-16 DOI:10.1016/j.isci.2026.117681

Kangyi Mu, Chunrong Ling, Tianlin Cui, Zequn Zhang, Chen Fang, Li Dong, Xiaosong Hu

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Abstract

Bacterial spores pose a significant challenge to the food industry due to their ability to germinate from dormancy and their extreme resistance. Spores are encased by a multilayered proteinaceous structure termed the spore coat. Previous studies on spore coat assembly have predominantly focused on the interactions among coat proteins, while the involvement of broader protein quality control systems remains poorly understood. Here, we demonstrate that the molecular chaperone DnaK is essential for the proper assembly of the inner spore coat in Bacillus subtilis. Deletion of dnaK leads to mislocalization of key morphogenetic protein SafA, reduced accumulation of full-length SafA, altered transcriptional dynamics of spoIIID and sigK, and ultimately impaired coat integrity and function. Our findings reveal that DnaK facilitates spore coat assembly through both transcriptional and post-translational mechanisms, uncovering a previously unrecognized regulatory layer in spore coat morphogenesis.

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伴侣蛋白dna介导的枯草芽孢杆菌孢子外壳组装调控。

细菌孢子对食品工业构成了重大挑战，因为它们具有从休眠状态萌发的能力和极强的抵抗力。孢子被称为孢子外壳的多层蛋白质结构所包裹。以前对孢子外壳组装的研究主要集中在外壳蛋白之间的相互作用上，而对更广泛的蛋白质质量控制系统的参与仍然知之甚少。在这里，我们证明了分子伴侣DnaK对于枯草芽孢杆菌内孢子外壳的正确组装是必不可少的。dnaK的缺失导致关键形态发生蛋白SafA的错误定位，减少全长SafA的积累，改变spoiid和sigK的转录动力学，最终损害外壳的完整性和功能。我们的研究结果表明，DnaK通过转录和翻译后机制促进孢子被组装，揭示了孢子被形态发生中一个以前未被识别的调控层。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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