Development, physicochemical characterization, and larvicidal efficacy of a novel Plectranthus barbatus Andrews solid formulation against Aedes aegypti L

IF 4.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2026-09-01 Epub Date: 2026-09-29 DOI:10.1016/j.bcab.2026.104265

Welton Aaron de Almeida, Livia Lais de Santana Silva, Cristiane Maria Varela de Araújo, Estefani Santana do Nascimento, Emerson de Oliveira Silva, Júlio César Ribeiro de Oliveira Farias de Aguiar, Lucas Gabriel Pita dos Santos, Patrícia Maria Guedes Paiva, Daniela Maria do Amaral Ferraz Navarro, Pedro José Rolim Neto, Thiago Henrique Napoleão, Emmanuel Viana Pontual

{"title":"Development, physicochemical characterization, and larvicidal efficacy of a novel Plectranthus barbatus Andrews solid formulation against Aedes aegypti L","authors":"Welton Aaron de Almeida, Livia Lais de Santana Silva, Cristiane Maria Varela de Araújo, Estefani Santana do Nascimento, Emerson de Oliveira Silva, Júlio César Ribeiro de Oliveira Farias de Aguiar, Lucas Gabriel Pita dos Santos, Patrícia Maria Guedes Paiva, Daniela Maria do Amaral Ferraz Navarro, Pedro José Rolim Neto, Thiago Henrique Napoleão, Emmanuel Viana Pontual","doi":"10.1016/j.bcab.2026.104265","DOIUrl":null,"url":null,"abstract":"<div><div>The increasing burden of Aedes aegypti-transmitted arboviruses highlights the need for innovative vector control strategies capable of overcoming the stability and dissolution limitations of botanical biolarvicides. However, the development and ecotoxicological evaluation of tablet-based formulations designed to improve the practical characteristics of botanical larvicides remain poorly explored. This study reports the development and ecotoxicological evaluation of a patented tablet formulation incorporating Plectranthus barbatus leaf extract. Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) demonstrated delayed thermal decomposition of the botanical matrix. Compatibility assessments by DSC and Fourier-transform infrared (FTIR) spectroscopy confirmed the absence of incompatibilities between the extract and selected excipients. The tablets exhibited rapid disintegration, with complete breakdown within 3 min 3 s under dynamic and 8.00 min under static conditions. In larvicidal bioassays against third-instar Ae. aegypti larvae, a single-dose tablet containing 0.48% (w/v) extract caused 40.0% mortality at 24 h and 66.2% at 48 h. Concentration–response modeling yielded an LC50 of 0.309% (w/v), lower than that of the crude extract (LC50 = 0.48%), with a diagnostic concentration of 0.34% (w/v). Ecotoxicological assays revealed acute sensitivity in Artemia salina (LC50 = 0.31% w/v at 24 h) and Tisbe biminiensis (LC50 = 0.65% w/v at 72 h). Additionally, 0.30% significantly delayed T. biminiensis development. Overall, the formulation retained larvicidal activity of P. barbatus and exhibited favorable thermal and disintegration characteristics under laboratory conditions. Further studies on storage stability, ecotoxicological effects, and semi-field or field performance are required before practical application.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104265"},"PeriodicalIF":4.6000,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocatalysis and agricultural biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878818126003488","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/9/29 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The increasing burden of Aedes aegypti-transmitted arboviruses highlights the need for innovative vector control strategies capable of overcoming the stability and dissolution limitations of botanical biolarvicides. However, the development and ecotoxicological evaluation of tablet-based formulations designed to improve the practical characteristics of botanical larvicides remain poorly explored. This study reports the development and ecotoxicological evaluation of a patented tablet formulation incorporating Plectranthus barbatus leaf extract. Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) demonstrated delayed thermal decomposition of the botanical matrix. Compatibility assessments by DSC and Fourier-transform infrared (FTIR) spectroscopy confirmed the absence of incompatibilities between the extract and selected excipients. The tablets exhibited rapid disintegration, with complete breakdown within 3 min 3 s under dynamic and 8.00 min under static conditions. In larvicidal bioassays against third-instar Ae. aegypti larvae, a single-dose tablet containing 0.48% (w/v) extract caused 40.0% mortality at 24 h and 66.2% at 48 h. Concentration–response modeling yielded an LC₅₀ of 0.309% (w/v), lower than that of the crude extract (LC₅₀ = 0.48%), with a diagnostic concentration of 0.34% (w/v). Ecotoxicological assays revealed acute sensitivity in Artemia salina (LC₅₀ = 0.31% w/v at 24 h) and Tisbe biminiensis (LC₅₀ = 0.65% w/v at 72 h). Additionally, 0.30% significantly delayed T. biminiensis development. Overall, the formulation retained larvicidal activity of P. barbatus and exhibited favorable thermal and disintegration characteristics under laboratory conditions. Further studies on storage stability, ecotoxicological effects, and semi-field or field performance are required before practical application.

查看原文本刊更多论文

一种新型barbatus Plectranthus Andrews固体制剂的研制、理化特性及对埃及伊蚊的杀灭效果

埃及伊蚊传播的虫媒病毒日益增加的负担突出表明，需要有创新的媒介控制策略，能够克服植物类生物杀虫剂的稳定性和溶解性限制。然而，开发和生态毒理学评价片剂配方旨在提高植物杀幼虫剂的实际特性仍然缺乏探索。本研究报道了一种含有barbatus叶提取物的专利片剂的开发和生态毒理学评价。热重分析（TGA）和差示扫描量热分析（DSC）证实了植物基质的延迟热分解。通过DSC和傅里叶变换红外（FTIR）光谱的相容性评估证实了提取物与所选辅料之间没有不相容。崩解速度快，动态条件下崩解时间为3 min 3 s，静态条件下崩解时间为8 min。对三龄伊蚊的杀幼虫生物测定。结果表明，含0.48% （w/v）提取物的单剂量片剂对埃及伊蚊幼虫24 h和48 h的死亡率分别为40.0%和66.2%。浓度响应模型的LC50为0.309% (w/v)，低于粗提取物（LC50 = 0.48%），诊断浓度为0.34% （w/v）。生态毒理学试验结果显示，青蒿素（24 h LC50 = 0.31% w/v）和天蚕（72 h LC50 = 0.65% w/v）急性敏感。此外，0.30%的添加量显著延缓了白桦尺蠖的发育。总体而言，该制剂保留了barbatus的杀幼虫活性，并在实验室条件下表现出良好的热降解特性。在实际应用之前，还需要进一步研究其储存稳定性、生态毒理学效应以及半田或田间性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.