{"title":"Advances in Low-Vacuum Scanning Electron Microscopy in Renal Pathological Diagnosis and Research.","authors":"Wanjun Shen, Fangxin Mu, Tiantian Wang","doi":"10.1002/jemt.70187","DOIUrl":null,"url":null,"abstract":"<p><p>Owing to its operational simplicity, low cost, and strong adaptability to a wide range of samples, low-vacuum scanning electron microscopy (LVSEM) has gradually become an indispensable tool in renal ultrastructural pathology. This article reviews the diverse applications of LVSEM in renal pathological diagnosis and research, including broad-area assessment of the glomerular basement membrane (GBM) surface architecture, analysis of crystal composition, and localization of immune complexes. The findings indicate that LVSEM holds significant diagnostic value in glomerular diseases, hereditary nephropathies, tubulointerstitial lesions, and kidney transplant pathology. LVSEM not only is an effective complement to traditional transmission electron microscopy (TEM) but also, through its large depth of field and capacity for surface-relief imaging, supports broader-area, quantitative, and multimodal ultrastructural analysis, with broad prospects for clinical application and research.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy Research and Technique","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/jemt.70187","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Owing to its operational simplicity, low cost, and strong adaptability to a wide range of samples, low-vacuum scanning electron microscopy (LVSEM) has gradually become an indispensable tool in renal ultrastructural pathology. This article reviews the diverse applications of LVSEM in renal pathological diagnosis and research, including broad-area assessment of the glomerular basement membrane (GBM) surface architecture, analysis of crystal composition, and localization of immune complexes. The findings indicate that LVSEM holds significant diagnostic value in glomerular diseases, hereditary nephropathies, tubulointerstitial lesions, and kidney transplant pathology. LVSEM not only is an effective complement to traditional transmission electron microscopy (TEM) but also, through its large depth of field and capacity for surface-relief imaging, supports broader-area, quantitative, and multimodal ultrastructural analysis, with broad prospects for clinical application and research.
期刊介绍：
Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
