A nuclear receptor PcHR96h regulates ABC transporters involved in spirodiclofen resistance in Panonychus citri.

IF 3.7 1区农林科学 Q1 ENTOMOLOGY

Insect Science Pub Date : 2026-10-09 DOI:10.1111/1744-7917.70373

Sichen Li, Shijiang Yu, Qiqi Yang, Zehao Huang, Yang Li, Mengxia Wang, Meiyi Huang, Shijun Fu, Zhen Wu, Liu Liu, Qi Pan, Wei Zhang, Haoqiang Liu, Lin Cong, Chun Ran

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引用次数: 0

Abstract

Transcription factors are known to regulate detoxification gene expression and contribute to resistance to insecticides and acaricides in arthropods. Our previous study revealed that the nuclear hormone receptor PcHR96h is associated with spirodiclofen resistance in Panonychus citri, but its regulatory mechanism remains unclear. Here, we used DAP-seq to identify genome-wide targets of PcHR96h and found that ABC transporter genes were significantly enriched. Dual-luciferase, yeast one-hybrid, and EMSA assays verified that PcHR96h directly binds to the promoters of PcABCA2 and PcABCB2. Functional validation via RNAi, subcellular localization, cytotoxicity tests, and molecular docking further confirmed that these two ABC transporters participate in spirodiclofen resistance. This study demonstrates that PcHR96h regulates specific ABC transporters involved in spirodiclofen resistance in P. citri, providing new insights into HR96-driven detoxification and acaricide resistance.

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核受体PcHR96h调控柑橘全爪虱抗螺双氯芬相关的ABC转运蛋白。

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来源期刊

Insect Science 生物-昆虫学

CiteScore

7.80

自引率

5.00%

发文量

1379

审稿时长

6.0 months

期刊介绍： Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.