Proteomic profiling reveals candidate biomarkers of ovarian inactivity in yak via Astral-DIA.

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2026-09-23 eCollection Date: 2026-01-01 DOI:10.3389/fvets.2026.1973877

Wanshu Wang, Yujun Li, Peng Wang, Min Ran, Yuehuan Zhang, Huizhu Zhang, Yaying Wang, Jiamei Luo, Bingjie Wang, Jian Li, Honghong He

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引用次数: 0

Abstract

Background: Inactive ovaries are one of the major causes of low reproductive performance in yaks, but their underlying molecular mechanisms remain unclear.

Methods: In this study, we applied the high‑throughput and high‑sensitivity Astral‑DIA (Data‑Independent Acquisition) technique to identify differentially expressed proteins in ovarian tissues from yaks with inactive ovaries (IO) compared to those in natural estrus (ECG).

Results: A total of 165 highly significant differentially expressed proteins were identified, including 87 upregulated and 78 downregulated proteins. Gene Ontology (GO), EggNOG functional classification, KEGG pathway analysis, and subcellular localization prediction were performed to annotate and characterize these proteins. Protein-protein interaction (PPI) network analysis revealed five representative differential proteins-MAO, FSTL3, HAPLN1, PTGFR, and RDH11-as potential key regulators of ovarian activity. Western blot validation confirmed the consistency of the proteomic data, showing that MAO, FSTL3, and HAPLN1 were significantly upregulated, while PTGFR and RDH11 were downregulated in the IO group.

Conclusion: These findings suggest that these five proteins may be involved in the pathogenesis of ovarian inactivity and could serve as biomarkers for early diagnosis and therapeutic targets. This study provides a theoretical foundation for further research on molecular mechanisms and prevention strategies for inactive ovaries in yaks.

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蛋白质组学分析通过Astral-DIA揭示了牦牛卵巢失活的候选生物标志物。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.