Detection of Legionella Bacteria Using a Silicon-Based Photonic Microring Resonator Biosensor Enhanced by Dielectrophoresis.

IF 2.6 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2026-10-09 DOI:10.1002/elps.70165

Anders Henriksson, Philipp Schrenk, Jamie R K Marland, Andreas Tsiamis, Ha Duong Ngo, Jörg König, David Schreier, Stewart Smith, Han Zhang, Arum Han, Peter Neubauer, Mario Birkholz

引用次数: 0

Abstract

Silicon-based photonic biosensors, such as microring resonators and Mach-Zehnder interferometers, offer label-free detection, high sensitivity, and full compatibility with semiconductor fabrication processes. Yet, the detection of living bacteria remains difficult due to the limited probability of cell binding on nanoscale waveguide surfaces. We present a microring resonator integrated with a nearby electrode that enables active cell transport via dielectrophoresis. Application of an alternating electric field concentrates cells within tens of micrometers of the sensing region, significantly enhancing detection efficiency. Using this approach, we demonstrate label-free detection of living Legionella bacteria. The method also enables future applications for distinguishing between live and dead cells, opening new possibilities for dynamic biological sensing.

查看原文本刊更多论文

介质电泳增强硅基光子微环谐振生物传感器检测军团菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.