A self-healing and injectable hydrogel for sustained delivery of BMSC-derived exosomes to promote osteogenesis and inhibit osteoclastogenesis in vitro

IF 2.1 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Bulletin of Materials Science Pub Date : 2026-09-30 DOI:10.1007/s12034-026-03774-6

Zhida Chen, Yangpeng Wu, Hanjie Zhuang, Yuanjie Jiang, Guofeng Huang, Taoyi Cai

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引用次数: 0

Abstract

The repair of osteoporotic bone defects remains highly challenging due to the pathological local microenvironment. Exosomes have shown potential as a cell-free therapeutic approach, but their rapid clearance in vivo limits their efficacy. In this study, we developed an injectable oxidized starch/gelatin composite hydrogel (GEL) based on dynamic Schiff base bonds as an intelligent sustained-release carrier for bone marrow mesenchymal stem cell-derived exosomes (Exos), aiming to provide a potential in vitro strategy for modulating bone metabolism. The hydrogel exhibits rapid gelation, notable shear-thinning, and self-healing properties. In vitro release studies demonstrated that the hydrogel enables sustained release of Exos for over 14 days. The exosome-loaded composite significantly enhanced the osteogenic differentiation of bone marrow mesenchymal stem cells and effectively suppressed RANKL-induced osteoclastogenesis. In summary, the GEL-Exos composite system constructed in this study provides a local and long-term bidirectional regulatory microenvironment for bone metabolism. Although in vivo validation is still needed, this system shows promise for future osteoporotic bone defect repair.

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一种自我修复的可注射水凝胶，用于持续递送骨髓间充质干细胞衍生的外泌体，以促进体外成骨和抑制破骨细胞的发生

由于局部病理微环境的原因，骨质疏松性骨缺损的修复仍然具有很高的挑战性。外泌体已经显示出作为一种无细胞治疗方法的潜力，但它们在体内的快速清除限制了它们的疗效。在这项研究中，我们开发了一种可注射的基于动态希夫碱键的氧化淀粉/明胶复合水凝胶（GEL），作为骨髓间充质干细胞衍生外泌体（Exos）的智能缓释载体，旨在提供一种潜在的体外调节骨代谢的策略。水凝胶具有快速凝胶化，显著的剪切变薄和自愈特性。体外释放研究表明，水凝胶可以使Exos持续释放超过14天。外泌体负载复合物显著增强骨髓间充质干细胞成骨分化，有效抑制rankl诱导的破骨细胞发生。综上所述，本研究构建的GEL-Exos复合体系为骨代谢提供了局部和长期的双向调控微环境。虽然还需要体内验证，但该系统显示了未来骨质疏松性骨缺陷修复的希望。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Materials Science 工程技术-材料科学：综合

CiteScore

3.40

自引率

5.60%

发文量

209

审稿时长

11.5 months

期刊介绍： The Bulletin of Materials Science is a bi-monthly journal being published by the Indian Academy of Sciences in collaboration with the Materials Research Society of India and the Indian National Science Academy. The journal publishes original research articles, review articles and rapid communications in all areas of materials science. The journal also publishes from time to time important Conference Symposia/ Proceedings which are of interest to materials scientists. It has an International Advisory Editorial Board and an Editorial Committee. The Bulletin accords high importance to the quality of articles published and to keep at a minimum the processing time of papers submitted for publication.