Lycopene partially ameliorates alcohol-induced sperm dysfunction and testicular toxicity in male Wistar rats

IF 3.1 Q2 MULTIDISCIPLINARY SCIENCES

Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2026-09-29 DOI:10.1186/s43088-026-00843-x

Oore-oluwapo Ololade Daramola, Adekunle Wahab Oyeyemi, Adeniran O. Akinola, Miracle Deborah Adeniyi, Ayomikun Joyce Tomomewo, Divine Ndako, Jesutofunmi Oguntimehin, Babatunde Oguntimehin, Dayo Rotimi Omotoso

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引用次数: 0

Abstract

Background

Alcohol consumption predisposes one to various hazards like reproductive toxicity. Lycopene, a carotenoid with antioxidant capacity credited with the ability to decrease oxidative stress has been reportedly used to ameliorate reproductive damage in experimental animals. This research investigated the mitigating abilities of lycopene administration on reproductive toxicity caused by alcohol in male Wistar rats.

Methods

Twenty male Wistar rats (150–180 g) were grouped as follows: 1 (control; 3 mL/kg 1% tween 80), 2 (Alcohol; Alc 3.2 g/kg of 30% v/v), 3 (lycopene; Lyc10 mg/kg) and 4 (Alc + Lyc). They were orally treated for 35 days and anaesthetized on day 36. Spermatozoa were profiled microscopically. 8-hydroxydeoxyguanosine, Caspase-3 activities, BcL-2 and interleukin-1β (IL-1β) were evaluated using Enzyme Linked Immunosorbent Assay (ELISA) technique. Malondialdehyde (MDA) concentrations, superoxide dismutase (SOD) and catalase were spectrophotometrically determined and testicular histopathology was assessed. Data derived was statistically analysed, expressed as Mean ± standard error of mean (Mean ± SEM) and compared using one way analysis of variance followed by Waller-Duncan’s post-hoc test.

Result

Alcohol caused an increase in abnormal morphology of spermatozoa. It also caused an increase in MDA concentration, Caspase-3 and IL-1β expression while a decrease in SOD, catalase activities and zinc level were observed. In addition, alcohol exposure caused histopathological changes within the testicular tissue. However, lycopene administration ameliorated all the observed deleterious changes induced by alcohol exposure.

Conclusion

Lycopene improved sperm morphology, testicular catalase activity and zinc level, lowered MDA level, Caspase 3 and interleukin 1 expression and reversed testicular histopathology in rats subjected to damages caused by alcohol.

Clinical trial number

Not applicable.

查看原文本刊更多论文

番茄红素部分改善雄性Wistar大鼠酒精诱导的精子功能障碍和睾丸毒性

饮酒会使人容易受到生殖毒性等各种危害。番茄红素是一种具有抗氧化能力的类胡萝卜素，被认为具有降低氧化应激的能力，据报道，它已被用于改善实验动物的生殖损伤。本研究探讨番茄红素对酒精致雄性Wistar大鼠生殖毒性的缓解作用。方法雄性Wistar大鼠20只（150 ~ 180 g），随机分为：1（对照组；80 ~ 80 % 1% 3 mL/kg）， 2（酒精；Alc 3.2 g/kg 30% v/v）， 3（番茄红素；Lyc10 mg/kg）， 4 （Alc + Lyc）。口服治疗35 d， 36 d麻醉。显微镜下观察精子。采用酶联免疫吸附法（ELISA）检测8-羟基脱氧鸟苷、Caspase-3活性、BcL-2和白细胞介素-1β (IL-1β)。分光光度法测定丙二醛（MDA）浓度、超氧化物歧化酶（SOD）和过氧化氢酶（过氧化氢酶），并评估睾丸组织病理学。对所得数据进行统计学分析，以均数±标准差（Mean±SEM）表示，并采用单向方差分析和沃勒-邓肯事后检验进行比较。结果酒精引起精子形态异常增加。MDA浓度、Caspase-3和IL-1β表达升高，SOD、过氧化氢酶活性和锌水平降低。此外，酒精暴露引起睾丸组织内的组织病理学改变。然而，番茄红素改善了所有观察到的由酒精暴露引起的有害变化。结论番茄红素能改善酒精损伤大鼠精子形态、睾丸过氧化氢酶活性和锌水平，降低丙二醛（MDA）水平、Caspase 3和白细胞介素1 （interleukin 1）表达，逆转睾丸组织病理学。临床试验编号不适用。

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来源期刊

Beni-Suef University Journal of Basic and Applied Sciences MULTIDISCIPLINARY SCIENCES-

CiteScore

2.60

自引率

0.00%

发文量

期刊介绍： Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.