First detection and molecular characterization of three trichomonad species in experimental beagle dogs in China.

IF 2.8 2区医学 Q2 PARASITOLOGY

Parasite Pub Date : 2026-01-01 Epub Date: 2026-10-02 DOI:10.1051/parasite/2026056

Xiao-Long Liao, Xue-Li Ge, Rong-Jun Gong, Jia-Min Ma, Yong-Hao Wang, Li-Qin Gui, Qing-Hong Guo, Xin-Chao Liu, Wen-Chao Li

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引用次数: 0

Abstract

Three different parasites of the phylum Parabasalia (Pentatrichomonas hominis, Tritrichomonas foetus and Dientamoeba fragilis) have been described in dogs. However, limited information about the prevalence of these trichomonad species in experimental beagle dogs in China is available. The aim of the current study was to investigate the frequency and molecular characterization of these three parasites species in these dogs. A total of 452 fecal samples were collected from ordinary clean-grade experimental beagle dogs and examined using PCR analysis, and positive samples were subsequently sequenced for species identification and analysis. Results showed that the prevalence of P. hominis, T. foetus and D. fragilis in the experimental dogs was 6.4% (29/452, 95% CI: 4.1-8.7%), 0% (0/452) and 0.7% (3/452, 95% CI: 0-1.4%), respectively. The sequence and phylogenetic analyses showed that the obtained sequences of P. hominis SSU rRNA genes were similar to the previously reported genotype CC1, and the sequences of D. fragilis SSU rRNA genes belonged to genotype 1, which is the most common genotype in humans. Our study provides the first data on the molecular prevalence of P. hominis, T. foetus and D. fragilis in experimental beagle dogs globally.

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中国实验比格犬中三种滴虫的首次检测及分子特征分析。

三种不同的副寄生虫门寄生虫（人五毛单胞菌、胎三毛单胞菌和脆弱地阿米巴）已在狗中被描述。然而，关于这些毛滴虫在中国实验比格犬中的流行情况的信息有限。本研究的目的是调查这三种寄生虫在这些狗身上的频率和分子特征。收集普通洁净级实验小猎犬粪便样本452份，采用PCR检测，阳性样本测序进行物种鉴定和分析。结果：实验犬中人弓形虫、胎儿弓形虫和脆弱弓形虫的患病率分别为6.4% （29/452,95% CI: 4.1 ~ 8.7%）、0%（0/452）和0.7% （3/452,95% CI: 0 ~ 1.4%）。序列分析和系统发育分析表明，获得的古人类假单胞虫SSU rRNA基因序列与先前报道的基因型CC1相似，而脆弱假单胞虫SSU rRNA基因序列属于人类最常见的基因型1。我们的研究首次提供了全球实验小猎犬中人类弓形虫、胎儿弓形虫和脆弱弓形虫分子流行率的数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.