Beyond Helicobacter pylori: Prevalence, Coinfection, and Clinicopathologic Patterns of Non-Helicobacter pylori Helicobacter (NHPH) in Human Gastric Biopsies

IF 4.6 2区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Helicobacter Pub Date : 2026-10-04 DOI:10.1111/hel.70165

Daniel Vizenblit, Maya Azrad, Munir Nashashibi, Halim Roshrosh, Yaakov Maman, Avi Peretz

{"title":"Beyond Helicobacter pylori: Prevalence, Coinfection, and Clinicopathologic Patterns of Non-Helicobacter pylori Helicobacter (NHPH) in Human Gastric Biopsies","authors":"Daniel Vizenblit, Maya Azrad, Munir Nashashibi, Halim Roshrosh, Yaakov Maman, Avi Peretz","doi":"10.1111/hel.70165","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Non-<i>Helicobacter pylori Helicobacter</i> (NHPH) species are increasingly recognized as human gastric colonizers, yet their prevalence, species distribution, and clinical significance remain incompletely defined, particularly in patient cohorts in the Middle East.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>In this retrospective study, 830 formalin-fixed paraffin-embedded gastric biopsy samples collected from patients undergoing esophagogastroduodenoscopy at Tzafon Medical Center, northern Israel, between 2022 and 2024 were subjected to species-specific PCR for <i>H. pylori</i> and NHPH species. Clinical, demographic, histopathological, and endoscopic data were correlated with PCR-defined infection status.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p><i>H. pylori</i> was detected in 643/830 (77.5%), whereas at least one NHPH species was detected in 179 samples (21.6%). <i>H. bizzozeronii</i> was the predominant NHPH species, identified in 167/830 samples (20.1%), while <i>H. heilmannii</i>, <i>H. felis</i>, and <i>H</i>. <i>ailurogastricus</i> were rare (< 1%), and <i>H. suis</i> and <i>H. salomonis</i> were not detected. Co-infection with <i>H. pylori</i> was common (18.2%), and was detected in 23.5% of all <i>H. pylori</i>-positive samples and 84.4% of NHPH-positive samples. NHPH prevalence was higher in females and demonstrated marked seasonality, peaking in autumn. Histopathological comparison showed a significant difference in foveolar hyperplasia rates across infection groups, with higher frequencies in NHPH-only and PCR detected negative for all species samples than in <i>H. pylori</i>-positive groups. In contrast, clinical symptoms and endoscopic findings were broadly comparable across infection strata.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>These findings provide the first large-scale molecular characterization of gastric NHPH in Israel and show that NHPH, predominantly <i>H. bizzozeronii</i>, is relatively common in this population, is usually co-detected with <i>H. pylori</i>, and is associated more strongly with distinct epidemiologic and histopathologic patterns than with a distinct clinical or endoscopic phenotype.</p>\n </section>\n </div>","PeriodicalId":13223,"journal":{"name":"Helicobacter","volume":"31 5","pages":""},"PeriodicalIF":4.6000,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13635466/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Helicobacter","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/hel.70165","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Non-Helicobacter pylori Helicobacter (NHPH) species are increasingly recognized as human gastric colonizers, yet their prevalence, species distribution, and clinical significance remain incompletely defined, particularly in patient cohorts in the Middle East.

Materials and Methods

In this retrospective study, 830 formalin-fixed paraffin-embedded gastric biopsy samples collected from patients undergoing esophagogastroduodenoscopy at Tzafon Medical Center, northern Israel, between 2022 and 2024 were subjected to species-specific PCR for H. pylori and NHPH species. Clinical, demographic, histopathological, and endoscopic data were correlated with PCR-defined infection status.

Results

H. pylori was detected in 643/830 (77.5%), whereas at least one NHPH species was detected in 179 samples (21.6%). H. bizzozeronii was the predominant NHPH species, identified in 167/830 samples (20.1%), while H. heilmannii, H. felis, and H. ailurogastricus were rare (< 1%), and H. suis and H. salomonis were not detected. Co-infection with H. pylori was common (18.2%), and was detected in 23.5% of all H. pylori-positive samples and 84.4% of NHPH-positive samples. NHPH prevalence was higher in females and demonstrated marked seasonality, peaking in autumn. Histopathological comparison showed a significant difference in foveolar hyperplasia rates across infection groups, with higher frequencies in NHPH-only and PCR detected negative for all species samples than in H. pylori-positive groups. In contrast, clinical symptoms and endoscopic findings were broadly comparable across infection strata.

Conclusions

These findings provide the first large-scale molecular characterization of gastric NHPH in Israel and show that NHPH, predominantly H. bizzozeronii, is relatively common in this population, is usually co-detected with H. pylori, and is associated more strongly with distinct epidemiologic and histopathologic patterns than with a distinct clinical or endoscopic phenotype.

查看原文本刊更多论文

超越幽门螺杆菌：人类胃活检中非幽门螺杆菌（NHPH）的患病率、共感染和临床病理模式。

背景：非幽门螺杆菌（Helicobacter pylori Helicobacter， NHPH）种越来越多地被认为是人类胃的定植菌，但它们的流行、种类分布和临床意义仍不完全明确，特别是在中东的患者群体中。材料和方法：本回顾性研究收集了以色列北部Tzafon医疗中心于2022年至2024年期间接受食管胃十二指肠镜检查的患者的830份福尔马林固定石蜡包埋胃活检样本，对幽门螺杆菌和NHPH进行了物种特异性PCR检测。临床、人口统计学、组织病理学和内窥镜数据与pcr定义的感染状态相关。结果：643/830份样本中检出幽门螺杆菌（77.5%），179份样本中检出至少一种NHPH（21.6%）。H. bizzozeronii为优势种，占167/830份（20.1%），H. heilmannii、H. felis和H. ailurogastricus为罕见种(结论：这些发现首次提供了以色列胃NHPH的大规模分子特征，并表明NHPH，主要是H. bizzozeronii，在该人群中相对常见，通常与幽门螺杆菌共同检测，并且与不同的流行病学和组织病理学模式的相关性比与不同的临床或内镜表型的相关性更强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Helicobacter 医学-微生物学

CiteScore

8.40

自引率

9.10%

发文量

审稿时长

2 months

期刊介绍： Helicobacter is edited by Professor David Y Graham. The editorial and peer review process is an independent process. Whenever there is a conflict of interest, the editor and editorial board will declare their interests and affiliations. Helicobacter recognises the critical role that has been established for Helicobacter pylori in peptic ulcer, gastric adenocarcinoma, and primary gastric lymphoma. As new helicobacter species are now regularly being discovered, Helicobacter covers the entire range of helicobacter research, increasing communication among the fields of gastroenterology; microbiology; vaccine development; laboratory animal science.