Biotope-dependent resistance to reactive oxygen species, antibiotic tolerance, and virulence of Staphylococcus aureus.

IF 5.8 1区生物学 Q1 BIOLOGY

Communications Biology Pub Date : 2026-10-01 DOI:10.1038/s42003-026-10771-2

Vincent Léguillier, Karine Gloux, Majd Khalife, Rochelle D'Mello, Zoran Minic, Milica Sentic, Marta Catto, Marisa Manzano, Christine Péchoux, Sandrine Truchet, Philippe Gaudu, Christina Nielsen-Leroux, Brahim Heddi, Alexandra Gruss, Jasmina Vidic

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Abstract

The human and livestock pathogen Staphylococcus aureus poses a major clinical challenge due to antibiotic treatment failure. Its resilience is mainly attributed to antibiotic resistance and tolerance mechanisms related to persistence. Here we investigate how two infection-relevant conditions, milk and serum, shape S. aureus pathogenic properties and capacity to withstand environmental stresses. Milk- versus serum-grown bacteria show gross differences in envelope physical properties, membrane fatty acid composition and rigidity, and pigment production, and display distinct proteomic profiles. Compared to growth in serum, S. aureus grown in milk exhibits extreme resistance to ROS damage, marked antimicrobial tolerance, and accelerated killing in a larval infection model. High level S. aureus pigmentation in whole milk is stimulated by milk lipids, and is responsible for high ROS resistance. The remarkable robustness of S. aureus in a milk biotope may signal the need to adjust antibiotic regimens when treating mastitis infections in humans and livestock.

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生物群落对活性氧的依赖抗性，抗生素耐受性和金黄色葡萄球菌的毒力。

由于抗生素治疗失败，人类和牲畜病原体金黄色葡萄球菌构成了一个重大的临床挑战。其恢复力主要归因于与持久性相关的抗生素耐药性和耐受性机制。在这里，我们研究了两种感染相关条件，牛奶和血清，如何塑造金黄色葡萄球菌的致病特性和承受环境压力的能力。牛奶培养的细菌与血清培养的细菌在包膜物理特性、膜脂肪酸组成和硬度、色素生产方面存在明显差异，并表现出不同的蛋白质组学特征。在幼虫感染模型中，与在血清中生长相比，在牛奶中生长的金黄色葡萄球菌对活性氧损伤表现出极强的抵抗力，具有明显的抗微生物耐受性，并加速杀死。高水平的金黄色葡萄球菌色素沉着在全脂牛奶是由牛奶脂刺激，并负责高活性氧抵抗。金黄色葡萄球菌在牛奶生物群落中的显著稳健性可能表明，在治疗人类和牲畜乳腺炎感染时，需要调整抗生素方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Communications Biology Medicine-Medicine (miscellaneous)

CiteScore

8.60

自引率

1.70%

发文量

1233

审稿时长

13 weeks

期刊介绍： Communications Biology is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the biological sciences. Research papers published by the journal represent significant advances bringing new biological insight to a specialized area of research.