E A Kurbatova, N F Gavrilova, I V Yakovleva, A E Zaytsev, An A Poddubikov, E S Petukhova, I B Semenova, Yu V Volokh, N O Vartanova
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引用次数: 0
Abstract
The hemolytic activity of pneumolysin produced by Streptococcus pneumoniae depends on the culture medium used for bacterial growth. In brain-heart infusion broth, both the culture medium and bacterial cell supernatants from all strains induced erythrocyte lysis and contained pneumolysin, which was detected using sandwich ELISA based on IgM and IgG1-HRP monoclonal antibodies to native pneumolysin. In contrast, samples obtained from strains grown on blood agar exhibited virtually no hemolytic activity, despite the presence of pneumolysin at high concentrations. These findings suggest that the culture medium affects the production of functionally active pneumolysin by pneumococcal strains. The developed sandwich ELISA based on monoclonal antibodies allows detection of pneumolysin that has lost its hemolytic properties.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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