Biological Effects of Secretory Phospholipase A₂ from Venoms of Gloydius blomhoffii and Gloydius halys.

IF 0.7 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2026-09-30 DOI:10.1007/s10517-026-06750-6

M A Solovyeva, L V Galebskaya, M A Galkin, E V Ryumina, G M Berdichevskiy, O S Shemchuk, E V Semenova, A V Ivanova, I M Mikhel, E A Rogozhin, L V Vasina

{"title":"Biological Effects of Secretory Phospholipase A2 from Venoms of Gloydius blomhoffii and Gloydius halys.","authors":"M A Solovyeva, L V Galebskaya, M A Galkin, E V Ryumina, G M Berdichevskiy, O S Shemchuk, E V Semenova, A V Ivanova, I M Mikhel, E A Rogozhin, L V Vasina","doi":"10.1007/s10517-026-06750-6","DOIUrl":null,"url":null,"abstract":"Gloydius blomhoffii and Gloydius halys venoms contain considerable amounts of group IIA secretory phospholipase (sPLA2). We studied the cytotoxic effects of some fractions from these venoms on the A549 lung adenocarcinoma cell line. G. blomhoffii venom fraction 16 and G. halys venom fraction 22 exhibited significant cytotoxicity against A549 cells (cell viability 10.7 ± 1.5% and 11.7 ± 2.7%, respectively). The sPLA2-sensitive melanoma SK-MEL-2 cell line and the sPLA2 inhibitor varespladib were used for sPLA2 identification and evaluation of its cytotoxicity in these fractions. Cell viability in the presence of the inhibitor increased by 26.4% (fraction 16) and 13% (fraction 22), which confirms a link between the cytotoxic effect and the enzymatic activity of sPLA2 in the fractions examined. The two protein components were further identified as acidic phospholipases A2.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-026-06750-6","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Gloydius blomhoffii and Gloydius halys venoms contain considerable amounts of group IIA secretory phospholipase (sPLA₂). We studied the cytotoxic effects of some fractions from these venoms on the A549 lung adenocarcinoma cell line. G. blomhoffii venom fraction 16 and G. halys venom fraction 22 exhibited significant cytotoxicity against A549 cells (cell viability 10.7 ± 1.5% and 11.7 ± 2.7%, respectively). The sPLA₂-sensitive melanoma SK-MEL-2 cell line and the sPLA₂ inhibitor varespladib were used for sPLA₂ identification and evaluation of its cytotoxicity in these fractions. Cell viability in the presence of the inhibitor increased by 26.4% (fraction 16) and 13% (fraction 22), which confirms a link between the cytotoxic effect and the enzymatic activity of sPLA₂ in the fractions examined. The two protein components were further identified as acidic phospholipases A₂.

查看原文本刊更多论文

黄颡鱼和黄颡鱼毒液分泌磷脂酶A2的生物学效应。

黄颡鱼（Gloydius blomhoffii）和黄颡鱼（Gloydius halys）毒液中含有大量的IIA族分泌磷脂酶（sPLA2）。我们研究了这些毒液的部分组分对A549肺腺癌细胞的细胞毒作用。黄颡鱼毒液16和黄颡鱼毒液22对A549细胞具有显著的细胞毒性（细胞活力分别为10.7±1.5%和11.7±2.7%）。sPLA2敏感性黑色素瘤SK-MEL-2细胞系和sPLA2抑制剂varespladib用于sPLA2鉴定和评估其在这些组分中的细胞毒性。在抑制剂存在下，细胞活力增加了26.4%（组分16）和13%（组分22），这证实了在所检测的组分中细胞毒性作用与sPLA2酶活性之间的联系。进一步鉴定这两种蛋白组分为酸性磷脂酶A2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.