Transplantation of undifferentiated and differentiated neural precursors towards Schwann-like phenotype in a model of acute spinal cord injury.

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2026-10-06 DOI:10.1016/j.advms.2026.10.002

Marcela María José Rodríguez-Baeza, Hermelinda Salgado-Ceballos, Nidia Jannette Carrillo-González, Tania Campos-Ordoñez, Laura Alvarez-Mejía, Gabriela Escobar-Camberos, Graciela Gudiño-Cabrera

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引用次数: 0

Abstract

Purpose: Traumatic spinal cord injury (TSCI) is a severe condition that profoundly affects individuals and places a substantial burden on healthcare systems. This study assesses functional recovery in TSCI under three conditions: a sham group without transplants, neural progenitor cells (NPCs) transplants, and NPCs differentiated into a Schwann-like phenotype (dNPCs) using an olfactory ensheathing cells-conditioned medium.

Materials/methods: An experimental model of TSCI was used, using complete transection in rats. Transplants were performed in the acute phase of TSCI using NPCs exposed to a conditioned medium.

Results: The NPCs formed neurospheres and acquired a Schwann-like morphology, confirmed via GFAP and p75 immunolabeling. NPCs-transplanted rats exhibited superior motor recovery compared to dNPCs and sham groups based on the Basso, Beattie, and Bresnahan (BBB) functional test. NPCs transplants promoted axonal regeneration and reduced lesion size. Both NPCs and dNPCs transplants reduced edema and inflammatory cell infiltration compared with the sham group. Immunohistochemistry revealed enhanced sensory neuron fiber regeneration (CGRP) and neuronal presence (NeuN) near the injury site in transplanted rats, with greater expression of p75 in NPCs-treated groups. Thus, the NPCs contributed to reduced lesion expansion, probably by releasing neurotrophic factors and forming a protective barrier.

Conclusions: These findings suggest that NPCs transplants may favorably modify the pathophysiological response to TSCI in the acute phase and improve functional outcomes, offering a potential therapeutic strategy for further investigation in chronic TSCI or its application in combined treatments.

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在急性脊髓损伤模型中，未分化和分化的神经前体向雪旺样表型移植。

目的：创伤性脊髓损伤（TSCI）是一种严重的疾病，深刻影响个人，并对医疗保健系统造成重大负担。本研究评估了三种情况下TSCI患者的功能恢复情况：无移植的假手术组、神经祖细胞（npc）移植组和使用嗅觉鞘细胞条件培养基分化为雪旺样表型（dNPCs）的npc。材料/方法：采用大鼠完全性脑损伤实验模型。移植在TSCI急性期使用暴露于条件培养基的npc进行。结果：经GFAP和p75免疫标记证实，NPCs形成神经球，呈雪旺样形态。根据Basso， Beattie和Bresnahan （BBB）功能测试，与dNPCs和假手术组相比，移植npcs的大鼠表现出更好的运动恢复。npc移植促进轴突再生，减小病变大小。与假手术组相比，NPCs和dNPCs移植均能减轻水肿和炎症细胞浸润。免疫组织化学显示，移植大鼠损伤部位附近感觉神经元纤维再生（CGRP）和神经元存在（NeuN）增强，npcs处理组p75表达增加。因此，NPCs可能通过释放神经营养因子和形成保护屏障来减少病变扩张。结论：这些研究结果表明，npc移植可能有利于改变急性期TSCI的病理生理反应，改善功能预后，为进一步研究慢性TSCI或其在联合治疗中的应用提供了潜在的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.