Synergistic sensitization between Rh-MOF and Ru(bpy)32+ driving superior electrochemiluminescence performance for selective L-proline detection

IF 5.4 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2026-10-07 DOI:10.1007/s00604-026-08431-7

Tasneem Omda Edrees Isaa, Tadele Eticha, Solomon Sime Tessema, Saima Hanif, Jiyang Liu, Cunqi Wu, Guobao Xu

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Abstract

A highly sensitive and cost-effective electrochemiluminescent (ECL) method has been developed for the selective detection of L-proline, addressing the growing demand for rapid small-molecule screening in health diagnostics. The method integrates a porous material, a rhodium-based metal–organic framework (Rh-MOF), synthesized via a rapid one-pot procedure involving coordination between Rh(III) ions and 2,4,6-tris(4-carboxyphenyl)-1,3,5-triazine (TCPrz), onto an isostatic graphite electrode (ISGE). Functioning as an efficient porous sensitizing host, Rh-MOF significantly amplified the anodic ECL signal of Ru(bpy)₃²⁺, resulting in a 93-fold enhancement. The method demonstrated excellent analytical performance, with a wide linear range (100 pM–1 mM), low detection limit (67 pM), and high selectivity toward L-proline. Analysis of real serum samples assured high recovery and good precision, highlighting the practical applicability of the proposed method in clinical diagnostics. This study offers novel perspectives on the development of MOFs as efficient enhancer, combined with the outstanding performance of low-cost ISGE, demonstrating promising applications in the detection of small biomolecules.

Graphical Abstract

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Rh-MOF和Ru(bpy)32+之间的协同敏化作用驱动了选择性l -脯氨酸检测的优越电化学发光性能

一种高灵敏度和高性价比的电化学发光（ECL）方法用于l-脯氨酸的选择性检测，满足了健康诊断中快速小分子筛选的需求。该方法将多孔材料铑基金属有机框架（Rh- mof）集成到等静力石墨电极（ISGE）上，通过Rh（III）离子与2,4,6-三（4-羧基苯基）-1,3,5-三嗪（TCPrz）之间的配位快速一锅法合成。作为一种高效的多孔增敏载体，Rh-MOF显著放大了Ru(bpy)32+的阳极ECL信号，增强了93倍。该方法线性范围宽（100 pM ~ 1 mM），检出限低（67 pM），对l -脯氨酸的选择性高。真实血清样品的分析保证了高回收率和良好的精密度，突出了该方法在临床诊断中的实用性。本研究为mof作为高效增强剂的发展提供了新的视角，结合低成本ISGE的出色性能，在小分子检测方面展示了广阔的应用前景。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.