Traffic-light-type visual sensing of bilirubin: a dual-emission Au-Ag NCs/MOF-808/Tb nanocomposite based on an asynchronous quenching strategy

IF 5.4 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2026-10-06 DOI:10.1007/s00604-026-08428-2

Jian Gou, Xinlu He, Luxin Xing, Ning Bi, Jun Xu, Lina Zhang, Yongxin Li, Yuanyuan Huang, Lei Jia

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Abstract

A dual-emission nanocomposite (Au-Ag NCs/MOF-808/Tb) was designed based on an asynchronous dual-quenching strategy. Gold-silver nanoclusters (Au-Ag NCs) with red fluorescence were modified on the surface of lanthanide-functionalized MOF-808, constructing a sensing system with traffic-light-like color transitions. Unlike the conventional ratiometric probes that rely on a stable internal reference, this system features two BR-responsive channels with different quenching rate (BR, bilirubin). Specifically, the green emission of Tb³⁺ at 545 nm showed a faster and more sensitive response toward BR, whereas the red emission of Au-Ag NCs at 650 nm changed more gently. On this basis, the 545 nm channel was selected as the primary analytical signal to establish the quantitative calibration curve, which afforded a limit of detection for BR as low as 44.24 nM.

The fluorescence intensity ratio I₅₄₅/I₆₅₀ was adopted to characterize the asynchronous response behavior of the dual-emission channels. Furthermore, by coupling the probe with a paper-based sensing platform and smartphone-assisted color analysis, visual and portable BR detection becomes possible with semi-quantitative concentration-range classification via a logic-gate scheme. By assembling multicomponent fluorescent materials with an asynchronous dual-response mechanism, this study demonstrates a new paradigm for the visual detection of biomarkers, and promotes the further development of portable analytical platforms.

Graphical Abstract

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红绿灯型胆红素视觉传感：基于异步猝灭策略的双发射Au-Ag NCs/MOF-808/Tb纳米复合材料

采用异步双猝灭策略，设计了Au-Ag NCs/MOF-808/Tb双发射纳米复合材料。将具有红色荧光的金-银纳米团簇（Au-Ag NCs）修饰在镧系功能化MOF-808表面，构建了具有红绿灯样颜色过渡的传感系统。与依赖稳定内参的传统比例探针不同，该系统具有两个不同猝灭速率的BR响应通道（BR，胆红素）。具体来说，Tb3+在545 nm处的绿色发射对BR的响应更快、更敏感，而Au-Ag NCs在650 nm处的红色发射变化更平缓。在此基础上，选择545 nm通道作为主要分析信号，建立定量校准曲线，对BR的检出限低至44.24 nm。采用荧光强度比I545/I650表征双发射通道的异步响应行为。此外，通过将探针与基于纸张的传感平台和智能手机辅助的颜色分析相结合，通过逻辑门方案进行半定量浓度范围分类，视觉和便携式BR检测成为可能。本研究通过组装具有异步双响应机制的多组分荧光材料，为生物标志物的视觉检测提供了新的范式，并促进了便携式分析平台的进一步发展。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.