Atorvastatin Prevents Early Lipotoxic Remodelling in Right Ventricular Pacing–Induced Cardiomyopathy

Abstract

Background

Right ventricular (RV) pacing can induce metabolic and structural alterations in left ventricular (LV) myocardium, including lipid accumulation, glycogen depletion, and increased endoplasmic reticulum (ER) stress. This study evaluated protective effects of atorvastatin on RV pacing–induced metabolic dysfunction.

Methods

Fifteen male Lanyu miniature pigs were randomized into sham control (n = 5), RV pacing (n = 5), and RV pacing with atorvastatin treatment (n = 5) groups. RV pacing was achieved via ventricular lead implantation at the RV outflow tract, followed by complete atrioventricular block, with ventricular pacing maintained for at least 80 beats/min in dual-chamber, dual-sensing, dual-response, rate-responsive mode to provide atrial-synchronous ventricular pacing while allowing atrial sensing or pacing. The RV pacing with atorvastatin treatment group received atorvastatin (40 mg/d) starting 2 weeks before surgery until euthanasia at 6 months. Metabolic, histologic, and molecular parameters were assessed in LV myocardial tissue.

Results

Compared with sham controls, RV pacing significantly increased myocardial lipid accumulation, with higher triglyceride (2.43 ± 0.17 vs 1.74 ± 0.14 μg/mg, P < 0.01) and diacylglycerol (2902 ± 567.7 vs 977.5 ± 182.5 μg/mg, P < 0.01) levels, and significantly reduced glycogen content (0.09 ± 0.03 vs 0.21 ± 0.03 μg/mg, P < 0.05). ER stress markers binding immunoglobulin protein and cleaved caspase-9 were markedly increased in the RV pacing group (P < 0.0001). Atorvastatin significantly reduced lipid accumulation (triglycerides: 1.83 ± 0.08 μg/mg; P < 0.05), ER stress, and apoptosis, but did not significantly restore glycogen levels.

Conclusions

RV pacing induces lipid accumulation, glycogen loss, and increased ER stress in the LV myocardium. Atorvastatin partially mitigated these effects by normalizing fatty acid metabolism and reducing ER stress and apoptosis, suggesting a potential role in attenuating early/subclinical RV pacing–induced metabolic remodelling.