Spins Afar─Long-Range Pulse Dipolar EPR Spectroscopy Uncovers Curvature in TRIM25’s Coiled Coil

IF 4.5 2区化学 Q2 CHEMISTRY, PHYSICAL

The Journal of Physical Chemistry Letters Pub Date : 2026-10-15 Epub Date: 2026-10-05 DOI:10.1021/acs.jpclett.6c02671

Yannik Limbach, Katrin Ackermann, Aisika Chakraborty, David G. Norman, Bela E. Bode

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引用次数: 0

Abstract

Pulse Dipolar Electron Paramagnetic Resonance Spectroscopy (PDS) is a powerful method for determining nanometer distances in biomolecular systems. This methodology has been successfully used to validate structure hypotheses and to identify disorder. However, inherent distance limitations are under-explored. Many challenging biomolecular applications require long distance information, driving the need for a benchmarking study. We demonstrate distances up to 10.5 nm in TRIM25 reliably extracted from data obtained under standard sample conditions. By protein perdeuteration accurate distance measurements up to 17.3 nm were enabled. Enhancing sensitivity through optimization of the measurement and sample preparation conditions the achievable dipolar evolution time was extended to 150 μs. Thus, distances close to 20 nm should become experimentally accessible, highlighting the potential of advanced PDS methodologies for probing previously inaccessible long-range structural information in complex systems.

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自旋远──远距脉冲偶极EPR光谱──覆盖TRIM25线圈的曲率

脉冲偶极电子顺磁共振波谱（PDS）是测定生物分子体系中纳米距离的一种有效方法。这种方法已成功地用于验证结构假设和识别紊乱。然而，固有的距离限制尚未得到充分探索。许多具有挑战性的生物分子应用需要远距离信息，这推动了对基准研究的需求。我们展示了在标准样品条件下获得的数据可靠地提取TRIM25的距离高达10.5 nm。通过蛋白质渗透，精确的距离测量可达17.3 nm。通过优化测量条件和制样条件，提高灵敏度，可实现的偶极演化时间延长至150 μs。因此，接近20纳米的距离应该可以在实验上实现，这突出了先进的PDS方法在探测复杂系统中以前无法获得的远程结构信息方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The Journal of Physical Chemistry Letters CHEMISTRY, PHYSICAL-NANOSCIENCE & NANOTECHNOLOGY

CiteScore

9.60

自引率

7.00%

发文量

1519

审稿时长

1.6 months

期刊介绍： The Journal of Physical Chemistry (JPC) Letters is devoted to reporting new and original experimental and theoretical basic research of interest to physical chemists, biophysical chemists, chemical physicists, physicists, material scientists, and engineers. An important criterion for acceptance is that the paper reports a significant scientific advance and/or physical insight such that rapid publication is essential. Two issues of JPC Letters are published each month.