High-throughput screening and genomic characterization of iron phosphate solubilizing bacteria for sustainable greenhouse production

IF 4.8 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2026-09-30 DOI:10.1007/s11104-026-09134-x

Sachin Naik,Juan Quijia-Pillajo,Laura J. Chapin,Michelle L. Jones

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Abstract

Abstract Background and aims Phosphorus availability is often limited in greenhouse production due to the formation of insoluble metal complexes in soilless substrates. Phosphate solubilizing bacteria (PSB) can break down these insoluble metal cation complexes and increase plant available forms of phosphorus. This study aims to identify and characterize iron PSB that enhance phosphorus availability in soilless greenhouse systems. Methods A collection of 1044 rhizobacterial isolates was screened for iron phosphate solubilization using a malachite green colorimetric assay. Isolates demonstrating greater than 25 percent solubilization of supplied FePO 4 ·2H 2 O were selected, sequenced, and classified taxonomically. These candidate strains were assessed in greenhouse trials under phosphorus limited conditions to evaluate plant growth promotion. Whole genome sequencing and functional annotation were performed to identify genes involved in phosphate solubilization and rhizosphere adaptation. Results Malachite green assay screening identified 24 high performing strains with strong FePO 4 ·2H 2 O solubilization ability. Greenhouse evaluation showed that Pantoea communis C3A8 , Pantoea formicae C8D10, Pseudomonas sp. C6E7, Pseudomonas sp. C9D1 and Priestia megaterium C3F10 improved spectral indicators of plant quality in French marigolds, while three of the strains (C3A8, C8D10, and C6E7) also increased shoot digital biomass. Strain C3A8 significantly increased shoot tissue P content. These strains encode unique genes supporting multiple phosphorus solubilization mechanisms as well as traits for effective rhizosphere colonization. Conclusion These findings highlight the importance of targeted screening and genomic analysis for identifying effective iron PSB strains, supporting the development of strategies aimed at reducing fertilizer inputs and promoting sustainable nutrient management in greenhouse crop production.

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可持续温室生产中磷酸铁增溶菌的高通量筛选和基因组学表征

摘要背景和目的由于在无土基质中形成不溶性金属配合物，在温室生产中磷的有效性经常受到限制。磷酸盐增溶菌（PSB）可以分解这些不溶性金属阳离子配合物，增加植物磷的有效形式。本研究旨在鉴定和表征在无土温室系统中提高磷有效性的铁PSB。方法采用孔雀石绿比色法对1044株根瘤菌进行磷酸铁增溶性筛选。对提供的fepo4·2h2o溶解力大于25%的分离株进行了筛选、测序和分类。这些候选菌株在限磷条件下进行了温室试验，以评价其对植物生长的促进作用。通过全基因组测序和功能注释，鉴定了参与磷酸盐溶解和根际适应的基因。结果孔雀石绿试验筛选出24株具有较强fepo4·2h2o溶解能力的高效菌株。温室评价结果表明，群落泛菌（Pantoea communis） C3A8、泛菌（Pantoea formicae） C8D10、假单胞菌（Pseudomonas sp. C6E7）、假单胞菌（Pseudomonas sp. C9D1）和巨花菊（Priestia megaterium C3F10）提高了法国金盏花植株品质光谱指标，其中3个菌株（C3A8、C8D10和C6E7）也提高了地上部数字生物量。菌株C3A8显著提高了茎部组织磷含量。这些菌株编码独特的基因，支持多种磷增溶机制以及有效定植根际的性状。结论这些发现突出了定向筛选和基因组分析对鉴定有效的铁PSB菌株的重要性，为制定减少肥料投入和促进温室作物生产可持续养分管理的策略提供了支持。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.