Assessment of acute functional effects of perfluorooctanoic acid (PFOA), heptafluorobutyric acid (HFBA), and hexafluoropropylene oxide dimer acid (HFPO-DA) in human iPSC-derived cardiomyocytes using multielectrode arrays

IF 4.6 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2026-10-01 Epub Date: 2026-10-02 DOI:10.1016/j.etap.2026.105184

Min Woo Kim, Myeonghee Lee, Jeong Hwan Park, Seul-Gi Lee, Jeong-Seop Oh, Shinhye Park, Youngin Jeong, Yoonseo Kim, Hyewon Shin, C-Yoon Kim, Hyung Min Chung

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引用次数: 0

Abstract

Per- and polyfluoroalkyl substances (PFASs) are persistent environmental pollutants associated with public health risks. The cardiac electrophysiological effects of the representative long-chain PFAS perfluorooctanoic acid (PFOA) and short-chain PFASs such as heptafluorobutyric acid (HFBA) and hexafluoropropylene oxide dimer acid (HFPO-DA) remain insufficiently understood. In this study, the electrophysiological effects of PFASs were evaluated in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) using multielectrode array (MEA) analysis. Exposure to 100 μM PFOA increased beat period and field potential duration, while reducing spike amplitude in a concentration-dependent manner without affecting contractility. In contrast, HFBA and HFPO-DA did not cause notable changes in either electrophysiological or contractility parameters. These results suggest that acute PFOA exposure disrupts cardiac electrophysiological function, whereas HFBA and HFPO-DA showed no notable effects within the tested conditions.

查看原文本刊更多论文

使用多电极阵列评估全氟辛酸（PFOA）、七氟丁酸（HFBA）和六氟环氧丙烷二聚酸（HFPO-DA）对人ipsc衍生心肌细胞的急性功能影响

全氟和多氟烷基物质（PFASs）是与公共健康风险相关的持久性环境污染物。代表性的长链全氟辛烷酸（PFOA）和短链全氟辛烷酸，如七氟丁酸（HFBA）和六氟环氧丙烷二聚酸（HFPO-DA）的心脏电生理效应仍未得到充分的了解。在本研究中，采用多电极阵列（MEA）分析方法评估了PFASs在人诱导多能干细胞源性心肌细胞（hiPSC-CMs）中的电生理效应。暴露在100 μM的PFOA中增加了心跳周期和场电位持续时间，同时以浓度依赖的方式降低了峰值振幅，而不影响收缩性。相比之下，HFBA和HFPO-DA均未引起电生理和收缩性参数的显著变化。这些结果表明，急性PFOA暴露会破坏心脏电生理功能，而HFBA和HFPO-DA在测试条件下没有显着影响。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.