Thiamine cross-feeding drives microbial coexistence in the leaf microbiome

IF 18.7 1区生物学 Q1 MICROBIOLOGY

Nature Microbiology Pub Date : 2026-10-05 DOI:10.1038/s41564-026-02499-w

Yiheng Hu, Johanna Bode, Daniel Gómez-Pérez, Marco Alexandre Guerreiro, Alfredo Mari, Kun Wang, Sinja Niemann, Maryam Mahmoudi, Ariane Kemen, Paloma Duran, Oskar Wacker, Daniel Straub, Sven Nahnsen, Benjamin Schwessinger, Fabrice Roux, Carlos Alonso-Blanco, Jon Ågren, Stéphane Hacquard, Eva H. Stukenbrock, Eric Kemen

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Abstract

Microbiome homeostasis is crucial for host health and ecosystem function, yet the molecular and ecological mechanisms underlying community assembly and stability remain elusive. Here we uncover a conserved yeast–oomycete association that promotes their coexistence in the leaf microbiome. Using a continental-scale microbiome survey, we identified an asymmetric mutualistic interaction between two eukaryotic hub microbes: the yeast Dioszegia hungarica and the obligate oomycete Albugo laibachii. We show that Dioszegia facilitates Albugo colonization by supplying thiamine via a dedicated membrane permease, alleviating Albugo’s auxotrophy. Genomic and transcriptomic analyses indicate that natural selection has acted on thiamine production in Dioszegia, shaping this metabolic complementation. In planta assays further suggest that Albugo presence supports Dioszegia persistence under glasshouse conditions. Our study illustrates how the evolution of nutrient cross-feeding mediates microbial coexistence and microbiome stability. Targeting microbial nutrient flows offers new strategies for engineering microbiomes and enhancing plant resilience in natural and agricultural systems.

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硫胺素交叉取食驱动微生物在叶片微生物群中的共存

微生物组的稳态对宿主健康和生态系统功能至关重要，但群落组装和稳定的分子和生态机制仍不清楚。在这里，我们发现了一个保守的酵母卵菌协会，促进他们共存的叶片微生物组。利用大陆尺度的微生物组调查，我们确定了两种真核中心微生物：酵母Dioszegia hungarica和专性卵菌Albugo laibachii之间的不对称互惠相互作用。我们表明，Dioszegia通过专门的膜渗透酶提供硫胺素，促进白蛋白定植，减轻白蛋白的营养不良。基因组学和转录组学分析表明，自然选择影响了硫胺的产生，形成了这种代谢互补。植物分析进一步表明，白蛋白的存在支持在温室条件下薯蓣的持久性。我们的研究阐明了营养物质交叉饲养的进化如何介导微生物共存和微生物组的稳定性。以微生物养分流动为目标为工程微生物组和增强自然和农业系统中的植物恢复力提供了新的策略。

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来源期刊

Nature Microbiology Immunology and Microbiology-Microbiology

CiteScore

44.40

自引率

1.10%

发文量

226

期刊介绍： Nature Microbiology aims to cover a comprehensive range of topics related to microorganisms. This includes: Evolution: The journal is interested in exploring the evolutionary aspects of microorganisms. This may include research on their genetic diversity, adaptation, and speciation over time. Physiology and cell biology: Nature Microbiology seeks to understand the functions and characteristics of microorganisms at the cellular and physiological levels. This may involve studying their metabolism, growth patterns, and cellular processes. Interactions: The journal focuses on the interactions microorganisms have with each other, as well as their interactions with hosts or the environment. This encompasses investigations into microbial communities, symbiotic relationships, and microbial responses to different environments. Societal significance: Nature Microbiology recognizes the societal impact of microorganisms and welcomes studies that explore their practical applications. This may include research on microbial diseases, biotechnology, or environmental remediation. In summary, Nature Microbiology is interested in research related to the evolution, physiology and cell biology of microorganisms, their interactions, and their societal relevance.