Carbohydrate-active enzymes from a core root mycobiota member enable infection of multiple plant hosts

IF 18.7 1区生物学 Q1 MICROBIOLOGY

Nature Microbiology Pub Date : 2026-10-01 DOI:10.1038/s41564-026-02492-3

Ram-Sevak Raja-Kumar, Fantin Mesny, Arpan Kumar Basak, Jacy Newfeld, Guillaume Chesneau, Frederickson Entila, Tak Lee, Linda Rigerte, Stephanie Carvajal Acevedo, Bruno Hüttel, Pedro W. Crous, Jose G. Maciá-Vicente, Helen Stewart, Matthew Ryan, Ahmad M. Fakhoury, Soledad Sacristán, Meriem Aitouguinane, Isabelle Batisson, Stefano Dumontet, Wade H. Elmer, Jana Henzelyová, Joanna S. Kruszewska, Jessica M. Nelson, Cara M. Santelli, Markus Pauly, Antonio Molina, Kei Hiruma, Stéphane Hacquard

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Abstract

The root microbiome includes fungal pathogens capable of colonizing multiple plant hosts, yet the underlying genetic determinants remain unknown. Here we report that Plectosphaerella cucumerina is a core member of the Arabidopsis thaliana root microbiota, which displays pathogenic potential across multiple hosts. Using a collection of 72 Plectosphaerella isolates and whole-genome sequencing, we observed subtle phenotypic and genotypic variation associated with fungal phylogeny but not host plant identity. Transcriptome profiling of a P. cucumerina isolate in roots of diverse plants revealed core and host-specific fungal responses, including induction of carbohydrate-active enzymes (CAZymes) involved in root cell wall deconstruction. A fungal gene encoding a candidate β-1,3-glucanase (GH64) was identified as a key genetic factor driving multihost infection. This gene is present across plant-colonizing fungi and functions as a disease determinant in both P. cucumerina and a Colletotrichum root pathogen. Our results indicate that host-induced CAZymes can couple fungal virulence with multihost compatibility.

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来自核心根真菌群成员的碳水化合物活性酶能够感染多个植物宿主

根系微生物群包括能够定植多个植物宿主的真菌病原体，但潜在的遗传决定因素仍然未知。本文报道黄瓜Plectosphaerella cucumerina是拟南芥（Arabidopsis thaliana）根系微生物群的核心成员，具有跨多个宿主致病的潜力。利用收集的72株Plectosphaerella分离株和全基因组测序，我们观察到与真菌系统发育相关的微妙表型和基因型变异，但与寄主植物身份无关。不同植物根中黄瓜假单胞菌的转录组分析揭示了核心和宿主特异性真菌反应，包括参与根细胞壁解构的碳水化合物活性酶（CAZymes）的诱导。一个真菌基因编码候选β-1,3-葡聚糖酶（GH64）被确定为驱动多宿主感染的关键遗传因素。该基因存在于植物定殖真菌中，并在黄瓜假根菌和炭疽菌根病原体中作为疾病决定因素发挥作用。我们的研究结果表明，宿主诱导的CAZymes可以将真菌毒力与多宿主相容性结合起来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Microbiology Immunology and Microbiology-Microbiology

CiteScore

44.40

自引率

1.10%

发文量

226

期刊介绍： Nature Microbiology aims to cover a comprehensive range of topics related to microorganisms. This includes: Evolution: The journal is interested in exploring the evolutionary aspects of microorganisms. This may include research on their genetic diversity, adaptation, and speciation over time. Physiology and cell biology: Nature Microbiology seeks to understand the functions and characteristics of microorganisms at the cellular and physiological levels. This may involve studying their metabolism, growth patterns, and cellular processes. Interactions: The journal focuses on the interactions microorganisms have with each other, as well as their interactions with hosts or the environment. This encompasses investigations into microbial communities, symbiotic relationships, and microbial responses to different environments. Societal significance: Nature Microbiology recognizes the societal impact of microorganisms and welcomes studies that explore their practical applications. This may include research on microbial diseases, biotechnology, or environmental remediation. In summary, Nature Microbiology is interested in research related to the evolution, physiology and cell biology of microorganisms, their interactions, and their societal relevance.