Role of mononuclear cells, stem cells, and exosomes in hypothyroid testicular dysfunction in rats via modulation of ZO-1, SIRT/FOXO. Focusing on blood testes barrier.
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引用次数: 0
Abstract
Bone marrow mesenchymal stem cells (BMSCs) are stromal cells that can self-renew. Exosomes (EXOs) perform many biological functions particularly in cell-cell communication. Peripheral blood mononuclear cells (PBMNCs) have the ability to differentiate into numerous mature functional cell types in specific microenvironments. This study aimed to compare the possible ameliorating effect of BMSCs, EXOs, and PBMNCs on hypothyroidism-induced testicular damage in adult male rats. Forty‑eight adult male albino rats were divided randomly into five groups: control, hypothyroidism (HT), BMSCs-treated, EXOs-treated, and PBMNCs-treated groups. Testes and thyroids were dissected and prepared for biochemical, histological, and immunohistochemical studies. After induction of HT, the results showed an apparent decrease in testes weight and abnormal semen analysis. Additionally, a significant decrease in antioxidant markers, thickness of the germinal epithelium, PAS staining, and testes BCL2 anti-apoptotic marker. It showed histological changes. Additionally, a significant decrease in SIRT-1 and ZO-1immune stained cells. A significant increase in testes BAX apoptotic marker, FOXO-1 immuno-expression, and deficient blood testes barrier with abnormal spermatogenic cells in electron microscopy were also detected. Improvement in all mentioned parameters in treated groups was noticed. Our study suggested that PBMNCs holds promise for alleviating hypothyroidism in rats on biochemical, histological, and immunohistochemical basis.
期刊介绍：
Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.
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