Transcriptome sequencing of Bacillus sp. HMM and effect of GH gene overexpression on cellulase activities.

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2026-09-29 DOI:10.1007/s10529-026-03795-5

Lanqian Huang, Su Liang, Shisong Yu, Xiyao Wang, Jianmei Ou, Qing Wang, Huili Huang, Chuzun Liao, Shiru Huang, Huiqing Zhang, Changhua Shang

引用次数: 0

Abstract

Bacillus sp. HMM can efficiently yield cellulase. However, transcriptome data and sequences of important genes such as cellulase gene are indistinct now, which restricts genetic reconstruction of strain HMM. In current paper, GO, COG and KEGG classification of unigenes from transcriptome sequencing of strain HMM was analyzed in detail, and cellulase gene GH (TR1834) encoding glycosylhydrolase was selected. Moreover, transgenic Bacillus sp. HMM overexpressing GH (GHT) with recombinant plasmid PHY-300PLK-GH was constructed. Avicel activity, CMC-Na activity and filter paper activity of GH transgenic HMM (GHT) were 1.4-, 1.2- and 1.08-fold those of control Bacillus sp. HMM with empty plasmid PHY-300PLK. This study enhanced application value of Bacillus for hydrolysis of cellulose.

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芽孢杆菌HMM转录组测序及GH基因过表达对纤维素酶活性的影响。

芽孢杆菌可高效产纤维素酶。然而，目前菌株的转录组数据和纤维素酶基因等重要基因的序列不明确，这限制了菌株的遗传重建。本文详细分析了HMM菌株转录组测序中单基因的GO、COG和KEGG分类，选择了编码糖基水解酶的纤维素酶基因GH （TR1834）。构建了以PHY-300PLK-GH重组质粒过表达GH （GHT）的转基因芽孢杆菌HMM。GH转基因HMM （GHT）的Avicel活性、CMC-Na活性和滤纸活性分别是空质粒PHY-300PLK对照芽孢杆菌HMM的1.4倍、1.2倍和1.08倍。本研究提高了芽孢杆菌在纤维素水解中的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.